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Literature summary for 3.2.1.37 extracted from

  • Pinphanichakarn, P.; Tangsakul, T.; Thongnumwon, T.; Talawanich, Y.; Thamchaipenet, A.
    Purification and characterization of ?-xylosidase from Streptomyces sp. CH7 and its gene sequence analysis. (2004), World J. Microbiol. Biotechnol., 20, 727-733.
    View publication on PubMed
Search for more literature for 3.2.1.37

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activates 1.2fold at 10 mM Streptomyces sp.

Cloned(Commentary)

Cloned (Comment) Organism
gene bxl2, ORF2, DNA and amino acid sequence determination and analysis Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 81% inhibition at 1 mM Streptomyces sp.
Cu2+ complete inhibition at 1 mM Streptomyces sp.
D-xylose competitive Streptomyces sp.
Fe2+ complete inhibition at 1 mM Streptomyces sp.
Hg2+ complete inhibition at 1 mM Streptomyces sp.
NEM 80% inhibition at 1 mM Streptomyces sp.
Ni2+ 95% inhibition at 1 mM Streptomyces sp.
Zn2+ complete inhibition at 1 mM Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.56
-
 4-nitrophenyl-beta-D-xylopyranoside pH 6.5, 55°C Streptomyces sp.
0.94
-
 2-nitrophenyl-beta-D-xylopyranoside pH 6.5, 55°C Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
87000
-
 2 * 87000, SDS-PAGE Streptomyces sp.
173000
-
 gel filtration Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp. Q7X508 gene bxl2
-
Streptomyces sp. CH7 Q7X508 gene bxl2
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 9.3fold from mycelium, by ammonium sulfate fractionation, 2 steps of ion exchange chromatography, and gel filtration Streptomyces sp.

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Streptomyces sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.3
-
 purified native enzyme Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-xylopyranoside + H2O
-
 Streptomyces sp. 2-nitrophenol + D-xylose
-
 ?
2-nitrophenyl beta-D-xylopyranoside + H2O
-
 Streptomyces sp. CH7 2-nitrophenol + D-xylose
-
 ?
4-nitrophenyl alpha-L-arabinofuranoside + H2O low activity Streptomyces sp. 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl alpha-L-arabinofuranoside + H2O low activity Streptomyces sp. CH7 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O best substrate Streptomyces sp. 4-nitrophenol + beta-D-xylose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O best substrate Streptomyces sp. CH7 4-nitrophenol + beta-D-xylose
-
 ?
xylan + H2O from oat spelt or birchwood, low activity Streptomyces sp. beta-D-xylose
-
 ?
xylan + H2O from oat spelt or birchwood, low activity Streptomyces sp. CH7 beta-D-xylose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 87000, SDS-PAGE Streptomyces sp.

Synonyms

Synonyms Comment Organism
beta-xylosidase
-
 Streptomyces sp.
xylanase
-
 Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, stable Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 30 min, stable Streptomyces sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
40
-
 D-xylose pH 6.5, 55°C, versus 4-nitrophenyl-beta-D-xylopyranoside Streptomyces sp.