Any feedback?
Literature summary for 3.2.1.37 extracted from
- Four types of beta-xylosidases from Penicillium wortmanni IFO 7237 (1987), Agric. Biol. Chem., 51, 2367-2379.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | 72-79% remaining activity for four isoenzymes | Penicillium wortmanni |  |
| Hg2+ | strong inhibition for all four isoenzymes | Penicillium wortmanni | |
| N-bromosuccinimide | complete inhibition for all four isoenzymes | Penicillium wortmanni | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
xylobiose | beta-xylosidase-3 | Penicillium wortmanni | |
| 2.3 | - |
xylobiose | beta-xylosidase-2 | Penicillium wortmanni | |
| 3.3 | - |
xylobiose | beta-xylosidase-4 | Penicillium wortmanni | |
| 4.2 | - |
xylobiose | beta-xylosidase-1 | Penicillium wortmanni | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Penicillium wortmanni | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
2 * 100000, xylosidase-4, SDS-PAGE | Penicillium wortmanni |
| 101000 | - |
2 * 101000, xylosidase-3, SDS-PAGE | Penicillium wortmanni |
| 105000 | - |
2 * 105000, xylosidase-2, SDS-PAGE | Penicillium wortmanni |
| 110000 | - |
xylosidase-1, SDS-PAGE, gel filtration | Penicillium wortmanni |
| 180000 | - |
xylosidase-4, SDS-PAGE, gel filtration | Penicillium wortmanni |
| 195000 | - |
xylosidase-2, SDS-PAGE, gel filtration | Penicillium wortmanni |
| 210000 | - |
xylosidase-3, SDS-PAGE, gel filtration | Penicillium wortmanni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xylan + H2O | Penicillium wortmanni | - |
beta-D-xylose + ? | - |
? | |
| xylobiose + H2O | Penicillium wortmanni | transxylosylation activity | beta-D-xylose + D-xylose | - |
? | |
| xylobiose + H2O | Penicillium wortmanni | four isoenzymes divided into three groups of action patterns | beta-D-xylose + D-xylose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium wortmanni | - |
four different isoenzymes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| separation of four isoenzymes to homogeneity | Penicillium wortmanni |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Penicillium wortmanni | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 22 | - |
beta-xylosidase-1 | Penicillium wortmanni |
| 30.5 | - |
beta-xylosidase-4 | Penicillium wortmanni |
| 40 | - |
beta-xylosidase-2 | Penicillium wortmanni |
| 61.2 | - |
beta-xylosidase-3 | Penicillium wortmanni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzyl-beta-D-xyloside + H2O | low activity for xylosidases-2,-3,-4, 45% activity for xylosidase-1 | Penicillium wortmanni | phenol + beta-D-xylose | - |
? | |
| butyl-beta-D-xyloside | low activity | Penicillium wortmanni | butanol + beta-D-xylose | - |
? | |
| cyclohexyl-beta-D-xyloside + H2O | 50% activity compared to that for xylobiose | Penicillium wortmanni | cyclohexanol + beta-D-xylose | - |
? | |
| methyl-beta-D-xyloside + H2O | very low activity | Penicillium wortmanni | beta-D-xylose + methanol | - |
? | |
| methyl-beta-D-xyloside + H2O | very low activity | Penicillium wortmanni | ? | - |
? | |
| p-nitrophenyl beta-D-xyloside + H2O | - |
Penicillium wortmanni | p-nitrophenol + beta-D-xylose | - |
? | |
| xylan + H2O | - |
Penicillium wortmanni | beta-D-xylose + ? | - |
? | |
| xylobiose + H2O | transxylosylation activity | Penicillium wortmanni | beta-D-xylose + D-xylose | - |
? | |
| xylobiose + H2O | four isoenzymes divided into three groups of action patterns | Penicillium wortmanni | beta-D-xylose + D-xylose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 101000, xylosidase-3, SDS-PAGE | Penicillium wortmanni |
| dimer | 2 * 105000, xylosidase-2, SDS-PAGE | Penicillium wortmanni |
| dimer | 2 * 100000, xylosidase-4, SDS-PAGE | Penicillium wortmanni |
| monomer | 1 * 110000, xylosidase-1, SDS-PAGE, gel filtration | Penicillium wortmanni |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-xylosidase-1 | - |
Penicillium wortmanni |
| beta-xylosidase-2 | - |
Penicillium wortmanni |
| beta-xylosidase-3 | - |
Penicillium wortmanni |
| beta-xylosidase-4 | - |
Penicillium wortmanni |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
beta-xylosidase-1 | Penicillium wortmanni |
| 60 | 65 | beta-xylosidase-3 | Penicillium wortmanni |
| 65 | - |
beta-xylosidases-2 and -4 | Penicillium wortmanni |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
stable up to for xylosidases-1,-3 and -4 | Penicillium wortmanni |
| 60 | - |
xylosidase-2, stable up to | Penicillium wortmanni |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 4 | beta-xylosidases-2,-3,-4 | Penicillium wortmanni |
| 4 | 4.5 | beta-xylosidase-1 | Penicillium wortmanni |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
