Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-D-xylose | 60% inhibition at 10 mM | Trichoderma viride | |
glucose | 16% inhibition at 10 mM | Trichoderma viride | |
Hg2+ | strong inhibition at 0.1 mM | Trichoderma viride | |
methyl-beta-D-xylopyranoside | 34% inhibition at 10 mM | Trichoderma viride |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
p-nitrophenyl beta-D-xylopyranoside | - |
Trichoderma viride | |
5 | - |
p-nitrophenyl beta-D-xylopyranoside | - |
Trichoderma viride |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Trichoderma viride | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | stimulation at 1 mM | Trichoderma viride | |
Mn2+ | stimulation at 1 mM | Trichoderma viride |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Trichoderma viride |
100000 | - |
gel filtration | Trichoderma viride |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylan + H2O | Trichoderma viride | limited capability to produce xylose due to synergistic action of xylanase and beta-xylosidase, synergistic action also needed for libration of acidic substituents | beta-D-xylose + ? | - |
? | |
xylo-oligosaccharides + H2O | Trichoderma viride | smaller xylooligosaccharides from 2-5mers less rapidly hydrolyzed | beta-D-xylose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma viride | - |
three isoforms | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 25% carbohydrate moiety | Trichoderma viride |
Purification (Comment) | Organism |
---|---|
to homogeneity by 2step chromatography | Trichoderma viride |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Trichoderma viride | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.4 | - |
- |
Trichoderma viride |
28.2 | - |
- |
Trichoderma viride |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl beta-D-xylopyranoside + H2O | - |
Trichoderma viride | p-nitrophenol + beta-D-xylopyranose | - |
? | |
xylan + H2O | limited capability to produce xylose due to synergistic action of xylanase and beta-xylosidase, synergistic action also needed for libration of acidic substituents | Trichoderma viride | beta-D-xylose + ? | - |
? | |
xylo-oligosaccharides + H2O | smaller xylooligosaccharides from 2-5mers less rapidly hydrolyzed | Trichoderma viride | beta-D-xylose + ? | - |
? | |
xyloheptaose + H2O | - |
Trichoderma viride | beta-D-xylose + ? | - |
? | |
xylohexaose + H2O | - |
Trichoderma viride | beta-D-xylose + ? | - |
? | |
xylooctaose + H2O | - |
Trichoderma viride | beta-D-xylose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE | Trichoderma viride |
Synonyms | Comment | Organism |
---|---|---|
beta-D-xyloside xylohydrolase | - |
Trichoderma viride |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Trichoderma viride |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
70% activity after 1 h | Trichoderma viride |
80 | - |
rapidly inactivated above | Trichoderma viride |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Trichoderma viride |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 7.5 | - |
Trichoderma viride |