General Stability | Organism |
---|---|
addition of Ca2+ protects against thermal inactivation | Malbranchea pulchella var. sulfurea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | strong inhibition | Malbranchea pulchella var. sulfurea | |
Hg2+ | complete inhibition | Aspergillus nidulans | |
Hg2+ | strong inhibition | Malbranchea pulchella var. sulfurea | |
Hg2+ | strong inhibition | Trichoderma viride | |
N-bromosuccinimide | complete inhibition at 1 mM | Aspergillus nidulans | |
N-bromosuccinimide | strong inhibition | Malbranchea pulchella var. sulfurea | |
N-bromosuccinimide | strong inhibition | Trichoderma viride | |
p-chloromercuribenzoate | strong inhibition | Aspergillus nidulans | |
p-chloromercuribenzoate | strong inhibition | Malbranchea pulchella var. sulfurea | |
SDS | complete inhibition | Aspergillus nidulans | |
SDS | strong inhibition | Malbranchea pulchella var. sulfurea | |
Zn2+ | strong inhibition | Malbranchea pulchella var. sulfurea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus nidulans | - |
- |
extracellular | - |
Trichoderma viride | - |
- |
extracellular | - |
Malbranchea pulchella var. sulfurea | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
gel filtration | Malbranchea pulchella var. sulfurea |
101000 | 102000 | SDS-PAGE, gel filtration | Trichoderma viride |
116000 | - |
2 * 116000, SDS-PAGE | Aspergillus nidulans |
240000 | - |
gel filtration, SDS-PAGE | Aspergillus nidulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylan + H2O | Malbranchea pulchella var. sulfurea | - |
beta-D-xylose + ? | - |
? | |
xylobiose + H2O | Aspergillus nidulans | - |
beta-D-xylose + D-xylose | - |
? | |
xylobiose + H2O | Trichoderma viride | - |
beta-D-xylose + D-xylose | - |
? | |
xylobiose + H2O | Malbranchea pulchella var. sulfurea | - |
beta-D-xylose + D-xylose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | - |
- |
- |
Malbranchea pulchella var. sulfurea | - |
- |
- |
Trichoderma viride | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 4% carbohydrate moiety | Aspergillus nidulans |
glycoprotein | 4.5% carbohydrate moiety | Trichoderma viride |
Purification (Comment) | Organism |
---|---|
to homogeneity by chromatography and electrophoretic steps | Malbranchea pulchella var. sulfurea |
to homogeneity by chromatography and isoelectric focusing | Trichoderma viride |
to homogeneity by chromatography, electrofocusing | Aspergillus nidulans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Aspergillus nidulans | - |
cell culture | - |
Trichoderma viride | - |
cell culture | - |
Malbranchea pulchella var. sulfurea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.5 | - |
- |
Malbranchea pulchella var. sulfurea |
10.8 | - |
- |
Trichoderma viride |
62.9 | - |
- |
Aspergillus nidulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyl-beta-D-xyloside + H2O | low activity | Aspergillus nidulans | phenol + beta-D-xylose | - |
? | |
butyl-beta-D-xyloside | low activity | Aspergillus nidulans | butanol + beta-D-xylose | - |
? | |
butyl-beta-D-xyloside | low activity | Trichoderma viride | butanol + beta-D-xylose | - |
? | |
cyclohexyl-beta-D-xyloside + H2O | low activity | Aspergillus nidulans | cyclohexanol + beta-D-xylose | - |
? | |
cyclohexyl-beta-D-xyloside + H2O | low activity | Trichoderma viride | cyclohexanol + beta-D-xylose | - |
? | |
methyl-beta-D-xyloside + H2O | very low activity | Trichoderma viride | beta-D-xylose + methanol | - |
? | |
methyl-beta-D-xyloside + H2O | very low activity | Trichoderma viride | ? | - |
? | |
o-nitrophenyl-beta-D-xyloside + H2O | - |
Aspergillus nidulans | o-nitrophenol + beta-D-xylose | - |
? | |
o-nitrophenyl-beta-D-xyloside + H2O | - |
Trichoderma viride | o-nitrophenol + beta-D-xylose | - |
? | |
p-nitrophenyl beta-D-xyloside + H2O | - |
Aspergillus nidulans | p-nitrophenol + beta-D-xylose | - |
? | |
p-nitrophenyl beta-D-xyloside + H2O | - |
Trichoderma viride | p-nitrophenol + beta-D-xylose | - |
? | |
p-nitrophenyl beta-D-xyloside + H2O | - |
Malbranchea pulchella var. sulfurea | p-nitrophenol + beta-D-xylose | - |
? | |
p-nitrophenyl-beta-D-xyloside + H2O | beta-xylosidase-1 | Malbranchea pulchella var. sulfurea | p-nitrophenol + alpha-D-xylose | inversion of anomeric configuration | ? | |
xylan + H2O | - |
Malbranchea pulchella var. sulfurea | beta-D-xylose + ? | - |
? | |
xylobiose + H2O | - |
Aspergillus nidulans | beta-D-xylose + D-xylose | - |
? | |
xylobiose + H2O | - |
Trichoderma viride | beta-D-xylose + D-xylose | - |
? | |
xylobiose + H2O | - |
Malbranchea pulchella var. sulfurea | beta-D-xylose + D-xylose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 116000, SDS-PAGE | Aspergillus nidulans |
monomer | 1 * 101000-102000, SDS-PAGE, gel filtration | Trichoderma viride |
monomer | 1 * 26000, SDS-PAGE, gel filtration | Malbranchea pulchella var. sulfurea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Malbranchea pulchella var. sulfurea |
55 | - |
- |
Aspergillus nidulans |
55 | - |
- |
Trichoderma viride |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable up to | Aspergillus nidulans |
50 | - |
50% activity retained after 15 min | Malbranchea pulchella var. sulfurea |
55 | - |
stable up to | Trichoderma viride |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
- |
Trichoderma viride |
4.5 | 5 | - |
Aspergillus nidulans |
6.2 | 6.8 | - |
Malbranchea pulchella var. sulfurea |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 4 | stable for 30 min at 50°C | Trichoderma viride |
4.5 | 5 | stable for 30 min at 50°C | Aspergillus nidulans |
6.3 | 6.7 | stable within | Malbranchea pulchella var. sulfurea |