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Literature summary for 3.2.1.37 extracted from
- Purification and characterization of beta-xylosidase from Aureobasidium pullulans (1991), Appl. Microbiol. Biotechnol., 35, 210-215.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Acetate buffer | increasing activity | Aureobasidium pullulans |  |
| dithiothreitol | increasing activity at 100 mM | Aureobasidium pullulans | |
General Stability
| General Stability | Organism |
|---|---|
| citrate phosphate and piperazine-HCl buffers reduce activity | Aureobasidium pullulans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | complete inactivation at 100 mM | Aureobasidium pullulans | |
| SDS | slight inhibition at 0.1%, w/v | Aureobasidium pullulans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.43 | - |
p-nitrophenyl beta-D-xyloside | - |
Aureobasidium pullulans | |
| 17.5 | - |
xylobiose | - |
Aureobasidium pullulans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aureobasidium pullulans | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 121000 | - |
2 * 121000, SDS-PAGE | Aureobasidium pullulans |
| 224000 | - |
gel filtration, SDS-PAGE | Aureobasidium pullulans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xylobiose + H2O | Aureobasidium pullulans | - |
beta-D-xylose + D-xylose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aureobasidium pullulans | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | N-glycosylated with high mannose structure | Aureobasidium pullulans |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity by 4step chromatography techniques | Aureobasidium pullulans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | highest enzyme production in xylan medium | Aureobasidium pullulans | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
- |
Aureobasidium pullulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl beta-D-xyloside + H2O | - |
Aureobasidium pullulans | p-nitrophenol + beta-D-xylose | - |
? | |
| xylobiose + H2O | - |
Aureobasidium pullulans | beta-D-xylose + D-xylose | - |
? | |
| xylotriose + H2O | also transxylosylation activity to xylotetraose | Aureobasidium pullulans | beta-D-xylose + xylobiose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 121000, SDS-PAGE | Aureobasidium pullulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Aureobasidium pullulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
27% loss of activity after 60 min | Aureobasidium pullulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Aureobasidium pullulans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 9.5 | stable within | Aureobasidium pullulans |
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