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Literature summary for 3.2.1.37 extracted from

  • Kumar, S.; Ramon, D.
    Purification and regulation of the synthesis of a beta-xylosidse from Aspergillus nidulans (1996), FEMS Microbiol. Lett., 135, 287-293.
No PubMed abstract available
Search for more literature for 3.2.1.37

Activating Compound

Activating Compound Comment Organism Structure
cysteine 30% stimulation Aspergillus nidulans
dithiothreitol 30% stimulation Aspergillus nidulans
EDTA 30% stimulation Aspergillus nidulans

General Stability

General Stability Organism
at optimal temperature inactivated within 4-6 h Aspergillus nidulans

Inhibitors

Inhibitors Comment Organism Structure
Ag+ strong inhibition Aspergillus nidulans
Cu2+ strong inhibition Aspergillus nidulans
Fe2+ some inhibition Aspergillus nidulans
Hg2+ strong inhibition Aspergillus nidulans
SDS some inhibition Aspergillus nidulans
Zn2+ some inhibition Aspergillus nidulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 p-nitrophenyl beta-D-xylopyranoside
-
 Aspergillus nidulans

Localization

Localization Comment Organism GeneOntology No. Textmining
cell bound
-
 Aspergillus nidulans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slight stimulation Aspergillus nidulans
Co2+ slightly stimulating Aspergillus nidulans
Mg2+ slight stimulation Aspergillus nidulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
 gel filtration Aspergillus nidulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylo-oligosaccharides + H2O Aspergillus nidulans derived from xylan beta-D-xylose + ?
-
 ?
xylobiose + H2O Aspergillus nidulans 90% degradation within 6 to 9 h beta-D-xylose + D-xylose
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 3step chromatography techniques Aspergillus nidulans

Source Tissue

Source Tissue Comment Organism Textmining
mycelium greatest level of enzyme in arabinose or xylose media Aspergillus nidulans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
107.1
-
-
 Aspergillus nidulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl beta-D-xylopyranoside + H2O
-
 Aspergillus nidulans p-nitrophenol + beta-D-xylopyranose
-
 ?
xylo-oligosaccharides + H2O derived from xylan Aspergillus nidulans beta-D-xylose + ?
-
 ?
xylobiose + H2O 90% degradation within 6 to 9 h Aspergillus nidulans beta-D-xylose + D-xylose
-
 ?
xylotetraose + H2O 20-30% degradation after 16 h Aspergillus nidulans xylotriose + beta-D-xylose
-
 ?
xylotriose + H2O 70-80% degradation after 16 h Aspergillus nidulans beta-D-xylose + xylobiose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 85000-95000, SDS-PAGE Aspergillus nidulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Aspergillus nidulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 45 stable up to 8 h, quite unstable above Aspergillus nidulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Aspergillus nidulans

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 6 stable up to 8 h at room temperature Aspergillus nidulans