Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-aminobenzimidazole | 0.04 mM, 92% inhibition | Bos taurus | |
6-chloromethyluracil | 0.05 mM, 94% inhibition | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | Q5E985 | - |
- |
Purification (Comment) | Organism |
---|---|
according to their inhibition activity for hyaluronidases, 6-chloromethyluracil and 2-aminobenzimidazole are coupled with sepharose beads for the affinity adsorption of bovine hyaluronidase-1. In good accordance with structural simulation, the ligands designed from 2-aminobenzimidazole show much higher specificity for the target enzyme, because they had more H bonds and pi-pi interactions with target sites. After optimization, through one step of affinity adsorption with sorbent L-4, bovine hyaluronidase can be isolated from the supernatant of bovine testes extract, with purity and recovery up to 96.4% and 89.4%, respectively. Based on the less steps of purification, short time consuming, higher purity, and higher activity recoveries, industrial application of this method is of great potential | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
testis | - |
Bos taurus | - |
Synonyms | Comment | Organism |
---|---|---|
hyaluronidase-1 | - |
Bos taurus |