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Literature summary for 3.2.1.35 extracted from
- Identification, characterization, and molecular cloning of a novel hyaluronidase, a member of glycosyl hydrolase family 16, from Penicillium spp. (2011), FEBS Lett., 585, 115-120.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | addition of MgCl2, CaCl2, and EDTA shows no significant effect | Talaromyces purpureogenus | |
| NaCl | 0.1-0.4 M NaCl increases the enzyme's activity by 20-30% | Talaromyces purpureogenus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Talaromyces purpureogenus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
PAGE | Talaromyces purpureogenus |
| 31700 | - |
1 * 30000, SDS-PAGE, 1 * 31700, calculated | Talaromyces purpureogenus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Talaromyces purpureogenus | E7FI87 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme | Talaromyces purpureogenus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
Talaromyces purpureogenus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hyaluronan + H2O | - |
Talaromyces purpureogenus | ? | catalyzes the endolytic hydrolysis of beta-1,4 glycosidic linkages in hyaluronan. Products are saturated hyaluronan-disaccharides | ? | |
| additional information | no substrate: chondroitin | Talaromyces purpureogenus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 30000, SDS-PAGE, 1 * 31700, calculated | Talaromyces purpureogenus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 43 | - |
- |
Talaromyces purpureogenus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 46 | more than 80% of maximum activity within | Talaromyces purpureogenus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
1 h, pH 3.0, more than 95% of initial activity | Talaromyces purpureogenus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 3.3 | - |
Talaromyces purpureogenus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.3 | - |
no activity above | Talaromyces purpureogenus |
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Release 2023.1 (January 2023)
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