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Literature summary for 3.2.1.35 extracted from

  • Kakizaki, I.; Ibori, N.; Kojima, K.; Yamaguchi, M.; Endo, M.
    Mechanism for the hydrolysis of hyaluronan oligosaccharides by bovine testicular hyaluronidase (2010), FEBS J., 277, 1776-1786.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Bos taurus
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Source Tissue

Source Tissue Comment Organism Textmining
testis
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Bos taurus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hyaluronan decasaccharide + H2O
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Bos taurus hyaluronan tetrasaccharide + hyaluronan hexasaccharide + hyaluronan octasaccharide + 31.5% hyaluronan tetrasaccharide, 48.3% hyaluronan hexasaccharide, 14.7% hyaluronan octasaccharide, 5.5% hyaluronan decasaccharide ?
hyaluronan octasaccharide + H2O
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Bos taurus hyaluronan tetrasaccharide + hyaluronan hexasaccharide 46.8% hyaluronan tetrasaccharide, 32.7% hyaluronan hexasaccharide, 20.5% hyaluronan octasaccharide. Enzyme generates a disaccharide intermediate from hyaluronan oligosaccharide, the majority of which is transferred to the nonreducing ends of other oligosaccharides, only traces being released as free disaccharide. When hyaluronan octasaccharide, with an unsaturated glucuronic acid at the nonreducing end, is used as a substrate, only a tetrasaccharide product is detected by HPLC. MS shows that the product is a mixture of equal amounts of two tetrasaccharides, one with and the other without the unsaturated glucuronic acid. This suggests that, in the case of substrates with a double bond at the nonreducing end, a tetrasaccharide is cleaved off instead of a disaccharide ?
additional information minimum substrate for bovine testicular hyaluronidase is the hyaluronan hexasaccharide Bos taurus ?
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Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
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Bos taurus