Cloned (Comment) | Organism |
---|---|
expressed in 22Rv1 human prostate adenomacarcinoma cells and in HEK-293 human embryonic kidney cells as a N-terminal FLAG fusion protein. Abundant soluble FLAG-tagged Hyal1 is secreted to the conditioned cell culture media of transfectants | Homo sapiens |
FLAG-tag | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D129N | catalytic mutant, catalysis impaired | Homo sapiens |
D129N | catalytic mutant, Km higher compared to wild-type, Vmax lower than wild-type | Homo sapiens |
DELTA351-end | putative structural mutant with a deleted EGF-like domain, significant decreased enzymatic activity | Homo sapiens |
DELTA356-end | putative structural mutant with a deleted EGF-like domain, significant decreased enzymatic activity | Homo sapiens |
E131Q | catalytic mutant, activity eliminated | Homo sapiens |
E131Q | catalytic mutant, no measurable activity | Homo sapiens |
L356tr | putative structural mutant, reduced activity | Homo sapiens |
N216A | putative structural mutant, reduced activity | Homo sapiens |
N216A | putative structural mutant, Km higher compared to wild-type, Vmax unaltered compared to wild-type, ca. 44% of wild-type activity | Homo sapiens |
N350A | putative structural mutant, reduced activity | Homo sapiens |
N350A | putative structural mutant, significant decreased enzymatic activity | Homo sapiens |
N350tr | putative structural mutant, reduced activity | Homo sapiens |
R265L | putative structural mutant, reduced activity | Homo sapiens |
R265L | putative structural mutant, significant decreased enzymatic activity | Homo sapiens |
S245A | substrate binding mutant, reduced activity | Homo sapiens |
S245A | substrate binding mutant, Km higher compared to wild-type, Vmax unaltered compared to wild-type, ca. 41% of wild-type activity | Homo sapiens |
Y202F | substrate binding mutant, catalysis impaired | Homo sapiens |
Y202F | substrate binding mutant, Km higher compared to wild-type, Vmax lower than wild-type | Homo sapiens |
Y247F | catalytic mutant, activity eliminated | Homo sapiens |
Y247F | catalytic mutant, no measurable activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
hyaluronan | Hyal1 wild-type | Homo sapiens | |
0.0381 | - |
hyaluronan | wild-type, Vmax: 12.5 micromol/min/mg | Homo sapiens | |
0.103 | - |
hyaluronan | Hyal1 mutant N216A | Homo sapiens | |
0.103 | - |
hyaluronan | mutant N216A, Vmax: 10 micromol/min/mg | Homo sapiens | |
0.11 | - |
hyaluronan | Hyal1 mutant S245A | Homo sapiens | |
0.11 | - |
hyaluronan | mutant S245A, Vmax: 10.7 micromol/min/mg | Homo sapiens | |
0.181 | - |
hyaluronan | Hyal1 mutant D129N | Homo sapiens | |
0.181 | - |
hyaluronan | mutant D129N, Vmax: 1.9 micromol/min/mg | Homo sapiens | |
0.367 | - |
hyaluronan | Hyal1 mutant Y202F | Homo sapiens | |
0.367 | - |
hyaluronan | mutant Y202F, Vmax: 6.7 micromol/min/mg | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | Q12794 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hyaluronan + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HYAL1 | - |
Homo sapiens |
hyaluronidase | - |
Homo sapiens |
hyaluronidase | isoenzyme Hyal1 | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.6 | - |
assay at | Homo sapiens |