Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.35 extracted from

  • Zhang, L.; Bharadwaj, A.G.; Casper, A.; Barkley, J.; Barycki, J.J.; Simpson, M.A.
    Hyaluronidase activity of human hyal1 requires active site acidic and tyrosine residues (2009), J. Biol. Chem., 284, 9433-9442.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in 22Rv1 human prostate adenomacarcinoma cells and in HEK-293 human embryonic kidney cells as a N-terminal FLAG fusion protein. Abundant soluble FLAG-tagged Hyal1 is secreted to the conditioned cell culture media of transfectants Homo sapiens
FLAG-tag Homo sapiens

Protein Variants

Protein Variants Comment Organism
D129N catalytic mutant, catalysis impaired Homo sapiens
D129N catalytic mutant, Km higher compared to wild-type, Vmax lower than wild-type Homo sapiens
DELTA351-end putative structural mutant with a deleted EGF-like domain, significant decreased enzymatic activity Homo sapiens
DELTA356-end putative structural mutant with a deleted EGF-like domain, significant decreased enzymatic activity Homo sapiens
E131Q catalytic mutant, activity eliminated Homo sapiens
E131Q catalytic mutant, no measurable activity Homo sapiens
L356tr putative structural mutant, reduced activity Homo sapiens
N216A putative structural mutant, reduced activity Homo sapiens
N216A putative structural mutant, Km higher compared to wild-type, Vmax unaltered compared to wild-type, ca. 44% of wild-type activity Homo sapiens
N350A putative structural mutant, reduced activity Homo sapiens
N350A putative structural mutant, significant decreased enzymatic activity Homo sapiens
N350tr putative structural mutant, reduced activity Homo sapiens
R265L putative structural mutant, reduced activity Homo sapiens
R265L putative structural mutant, significant decreased enzymatic activity Homo sapiens
S245A substrate binding mutant, reduced activity Homo sapiens
S245A substrate binding mutant, Km higher compared to wild-type, Vmax unaltered compared to wild-type, ca. 41% of wild-type activity Homo sapiens
Y202F substrate binding mutant, catalysis impaired Homo sapiens
Y202F substrate binding mutant, Km higher compared to wild-type, Vmax lower than wild-type Homo sapiens
Y247F catalytic mutant, activity eliminated Homo sapiens
Y247F catalytic mutant, no measurable activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
hyaluronan Hyal1 wild-type Homo sapiens
0.0381
-
hyaluronan wild-type, Vmax: 12.5 micromol/min/mg Homo sapiens
0.103
-
hyaluronan Hyal1 mutant N216A Homo sapiens
0.103
-
hyaluronan mutant N216A, Vmax: 10 micromol/min/mg Homo sapiens
0.11
-
hyaluronan Hyal1 mutant S245A Homo sapiens
0.11
-
hyaluronan mutant S245A, Vmax: 10.7 micromol/min/mg Homo sapiens
0.181
-
hyaluronan Hyal1 mutant D129N Homo sapiens
0.181
-
hyaluronan mutant D129N, Vmax: 1.9 micromol/min/mg Homo sapiens
0.367
-
hyaluronan Hyal1 mutant Y202F Homo sapiens
0.367
-
hyaluronan mutant Y202F, Vmax: 6.7 micromol/min/mg Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens Q12794
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hyaluronan + H2O
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
HYAL1
-
Homo sapiens
hyaluronidase
-
Homo sapiens
hyaluronidase isoenzyme Hyal1 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.6
-
assay at Homo sapiens