Cloned (Comment) | Organism |
---|---|
isozymes are clustered on chromosome 9F1-F2 and 6A2, expression profiles | Mus musculus |
isozymes Hyal1-Hyal4, sequence comparison, the genes encoding isozymes Hyal1-Hyal3 are tightly clustered on chromosome 3q21.3, Hyal4 and HPH-20 are clustered on 7q31.3, expression profiles | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
three-dimensional structure analysis | Bos taurus |
three-dimensional structure analysis | Apis mellifera |
Protein Variants | Comment | Organism |
---|---|---|
D146N | site-directed mutagenesis of HPH-20, the mutant shows highly reduced activity | Homo sapiens |
E148Q | site-directed mutagenesis of HPH-20, inactive mutant | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
acrosome | spermatid, HPH-20 | Homo sapiens | - |
- |
extracellular | venom | Apis mellifera | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hyaluronan + H2O | Mus musculus | - |
hyaluronan oligomers | - |
? | |
hyaluronan + H2O | Bos taurus | - |
hyaluronan oligomers | - |
? | |
hyaluronan + H2O | Apis mellifera | - |
hyaluronan oligomers | - |
? | |
hyaluronan + H2O | Homo sapiens | the enzyme is required for fertility facilitating the penetration of sperm through the cumulus mass in the ovum | hyaluronan oligomers | - |
? | |
additional information | Homo sapiens | HPH-20 or SPAM1, sperm adhesion molecule 1, is a bifunctional enzyme, which is also an adhesion molecule with binding properties to the cumulus mass surrounding the ovum | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Apis mellifera | Q08169 | - |
- |
Bos taurus | - |
- |
- |
Homo sapiens | - |
isozymes Hyal1-Hyal4, and HPH-20, or SPAM1 | - |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
hyaluronic acid hexasaccharide + H2O = hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide | reaction mechanism and three-dimensional structure | Mus musculus | |
hyaluronic acid hexasaccharide + H2O = hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide | reaction mechanism and three-dimensional structure, catalytic residue is Glu113, substrate positioning residues, overview | Apis mellifera | |
hyaluronic acid hexasaccharide + H2O = hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide | reaction mechanism and three-dimensional structure, catalytic residue is Glu149, substrate positioning residues, overview | Bos taurus | |
hyaluronic acid hexasaccharide + H2O = hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide | reaction mechanism and three-dimensional structure, catalytic residues of isozymes Hyal1-Hyal4 are Glu131, Glu135, Glu129, and Glu147, respectively, substrate positioning residues, active site structure of isozymes, overview | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
breast | HPH-20 | Homo sapiens | - |
commercial preparation | testicular | Bos taurus | - |
epididymis | HPH-20 | Homo sapiens | - |
ovary | HPH-20 | Homo sapiens | - |
spermatid | HPH-20 | Homo sapiens | - |
spermatozoon | - |
Homo sapiens | - |
testis | - |
Bos taurus | - |
testis | HPH-20 | Homo sapiens | - |
uterus | HPH-20 | Homo sapiens | - |
venom | - |
Apis mellifera | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
commercial preparation | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hyaluronan + H2O | - |
Mus musculus | hyaluronan oligomers | - |
? | |
hyaluronan + H2O | - |
Homo sapiens | hyaluronan oligomers | - |
? | |
hyaluronan + H2O | - |
Bos taurus | hyaluronan oligomers | - |
? | |
hyaluronan + H2O | - |
Apis mellifera | hyaluronan oligomers | - |
? | |
hyaluronan + H2O | the enzyme is required for fertility facilitating the penetration of sperm through the cumulus mass in the ovum | Homo sapiens | hyaluronan oligomers | - |
? | |
additional information | HPH-20 or SPAM1, sperm adhesion molecule 1, is a bifunctional enzyme, which is also an adhesion molecule with binding properties to the cumulus mass surrounding the ovum | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | three-dimensional structure analysis | Bos taurus |
More | three-dimensional structure analysis | Apis mellifera |
More | three-dimensional structure analysis and modeling of isozymes, structure-reaction mechanism relationship, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
BPH-20 | - |
Bos taurus |
HPH-20 | - |
Homo sapiens |
human PH-20 | - |
Homo sapiens |
Hyal | - |
Homo sapiens |
hyaluronidase | - |
Mus musculus |
hyaluronidase | - |
Homo sapiens |
hyaluronidase | - |
Bos taurus |
hyaluronidase | - |
Apis mellifera |
SPAM1 | - |
Homo sapiens |
spreading factor | - |
Homo sapiens |