Application | Comment | Organism |
---|---|---|
medicine | enzyme is the target for immunotherapy of allergen reaction | Apis mellifera |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence analysis, expression as active C-terminally His-tagged enzyme in insect cells via baculovirus infection | Apis mellifera |
Crystallization (Comment) | Organism |
---|---|
8-10 mg/ml purified recombinant enzyme, with or wihtout hexameric hyaluronic acid, in 5 mM sodium acetate, pH 5.4, hanging drop vapour diffusion method, 20°C, for triclinic crystals, equal volume of protein solution with reservoir solution containing 30% PEG 8000, 0.1 M sodium cacodylate, pH 6.5, 0.2 M ammonium sulfate, equilibration against the reservoir solution, 6-8 months, for monoclinic crystal growth within several days, 10% PEG 6000, 1.0 M NaCl, 20 mM acetic acid, pH 4.2 is used for mixing and equilibration, X-ray diffraction structure determination and analysis at 1.6-2.7 A resolution | Apis mellifera |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Apis mellifera | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hyaluronate + H2O | Apis mellifera | - |
N-acetyl-beta-D-glucosamine + D-glucuronate + ? | - |
? | |
additional information | Apis mellifera | the enzyme is a major allergen of bee venom which can induce serious, occasionally fatal, systemic IgE-mediated anaphylactic reactions in humans | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Apis mellifera | Q08169 | bee | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme | Apis mellifera |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
hyaluronic acid hexasaccharide + H2O = hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide | enzyme is a beta-1,4-glucosidase, substrate binding groove, enzyme-substrate complex structure, catalytic groups and reaction mechanism | Apis mellifera |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Apis mellifera | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hyaluronate + H2O | - |
Apis mellifera | N-acetyl-beta-D-glucosamine + D-glucuronate + ? | - |
? | |
additional information | the enzyme is a major allergen of bee venom which can induce serious, occasionally fatal, systemic IgE-mediated anaphylactic reactions in humans | Apis mellifera | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Hya | - |
Apis mellifera |
hyaluronidase | - |
Apis mellifera |