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Literature summary for 3.2.1.35 extracted from

  • Markovic-Housley, Z.; Miglierini, G.; Soldatova, L.; Rizkallah, P.J.; Muller, U.; Schirmer, T.
    Crystal structure of hyaluronidase, a major allergen of bee venom (2000), Structure, 8, 1025-1035.
    View publication on PubMed

Application

Application Comment Organism
medicine enzyme is the target for immunotherapy of allergen reaction Apis mellifera

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence analysis, expression as active C-terminally His-tagged enzyme in insect cells via baculovirus infection Apis mellifera

Crystallization (Commentary)

Crystallization (Comment) Organism
8-10 mg/ml purified recombinant enzyme, with or wihtout hexameric hyaluronic acid, in 5 mM sodium acetate, pH 5.4, hanging drop vapour diffusion method, 20°C, for triclinic crystals, equal volume of protein solution with reservoir solution containing 30% PEG 8000, 0.1 M sodium cacodylate, pH 6.5, 0.2 M ammonium sulfate, equilibration against the reservoir solution, 6-8 months, for monoclinic crystal growth within several days, 10% PEG 6000, 1.0 M NaCl, 20 mM acetic acid, pH 4.2 is used for mixing and equilibration, X-ray diffraction structure determination and analysis at 1.6-2.7 A resolution Apis mellifera

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Apis mellifera
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hyaluronate + H2O Apis mellifera
-
N-acetyl-beta-D-glucosamine + D-glucuronate + ?
-
?
additional information Apis mellifera the enzyme is a major allergen of bee venom which can induce serious, occasionally fatal, systemic IgE-mediated anaphylactic reactions in humans ?
-
?

Organism

Organism UniProt Comment Textmining
Apis mellifera Q08169 bee
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme Apis mellifera

Reaction

Reaction Comment Organism Reaction ID
hyaluronic acid hexasaccharide + H2O = hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide enzyme is a beta-1,4-glucosidase, substrate binding groove, enzyme-substrate complex structure, catalytic groups and reaction mechanism Apis mellifera

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Apis mellifera
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hyaluronate + H2O
-
Apis mellifera N-acetyl-beta-D-glucosamine + D-glucuronate + ?
-
?
additional information the enzyme is a major allergen of bee venom which can induce serious, occasionally fatal, systemic IgE-mediated anaphylactic reactions in humans Apis mellifera ?
-
?

Synonyms

Synonyms Comment Organism
Hya
-
Apis mellifera
hyaluronidase
-
Apis mellifera