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Literature summary for 3.2.1.35 extracted from

  • Takahashi, T.; Ikegami-Kawai, M.; Okuda, R.; Suzuki, K.
    A fluorometric Morgan-Elson method for hyaluronidase activity (2003), Anal. Biochem., 322, 257-263.
    View publication on PubMed
Search for more literature for 3.2.1.35

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Homo sapiens
additional information
-
 additional information kinetics Bos taurus
additional information
-
 additional information kinetics Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl
-
 Homo sapiens
NaCl
-
 Bos taurus
NaCl
-
 Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens
-
-
-
Oryctolagus cuniculus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
hyaluronic acid hexasaccharide + H2O = hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide also hydrolyses 1,4-beta-D-glycosidic linkages between N-acetyl-galactosamine or N-acetylgalactosamine sulfate and glucuronic acid in chondroitin, chondroitin 4- and 6-sulfates, and dermatan Homo sapiens
a
hyaluronic acid hexasaccharide + H2O = hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide also hydrolyses 1,4-beta-D-glycosidic linkages between N-acetyl-galactosamine or N-acetylgalactosamine sulfate and glucuronic acid in chondroitin, chondroitin 4- and 6-sulfates, and dermatan Bos taurus
a
hyaluronic acid hexasaccharide + H2O = hyaluronic acid disaccharide + hyaluronic acid tetrasaccharide also hydrolyses 1,4-beta-D-glycosidic linkages between N-acetyl-galactosamine or N-acetylgalactosamine sulfate and glucuronic acid in chondroitin, chondroitin 4- and 6-sulfates, and dermatan Oryctolagus cuniculus
a

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Bos taurus
-
serum
-
 Homo sapiens
-
serum
-
 Oryctolagus cuniculus
-
testicle
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity in serum samples, development of a highly sensitive fluorometric assay method using a chromogenic reaction with the N-acetylglucosamine residues at the reducing ends produced by the enzymic cleavage, mechanism, overview Homo sapiens
additional information
-
 activity in serum samples, development of a highly sensitive fluorometric assay method using a chromogenic reaction with the N-acetylglucosamine residues at the reducing ends produced by the enzymic cleavage, mechanism, overview Oryctolagus cuniculus
additional information
-
 development of a highly sensitive fluorometric assay method using a chromogenic reaction with the N-acetylglucosamine residues at the reducing ends produced by the enzymic cleavage, mechanism, overview Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hyaluronan + 2 H2O from Streptococcus zooepidemicus, degradation Homo sapiens N-acetyl-beta-D-glucosamine + D-glucuronate + ?
-
 ?
hyaluronan + 2 H2O from Streptococcus zooepidemicus, degradation Bos taurus N-acetyl-beta-D-glucosamine + D-glucuronate + ?
-
 ?
hyaluronan + 2 H2O from Streptococcus zooepidemicus, degradation Oryctolagus cuniculus N-acetyl-beta-D-glucosamine + D-glucuronate + ?
-
 ?

Synonyms

Synonyms Comment Organism
HAase
-
 Bos taurus
hyaluronidase
-
 Homo sapiens
hyaluronidase
-
 Bos taurus
hyaluronidase
-
 Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens
37
-
 assay at Bos taurus
37
-
 assay at Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.9
-
 assay at Oryctolagus cuniculus
3.9
-
 assay at Homo sapiens
6
-
 assay at Bos taurus