Any feedback?
Literature summary for 3.2.1.35 extracted from
- Hyaluronidases (1952), Adv. Enzymol. Relat. Subj. Biochem., 13, 199-236.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | 60% activation | Bos taurus |  |
| NaCl | - |
Homo sapiens | |
| NaCl | prevents inhibition by impurities present in the substrate | Bos taurus | |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | increasing absorption of fluids in dermal clysis, increasing diffusion of injected substances | Bos taurus |
General Stability
| General Stability | Organism |
|---|---|
| at room temperature activity rapidly lost, addition of a peptide derived from gelatin increases stability | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetylated hyaluronic acid | - |
Bos taurus | |
| Cu2+ | inhibition reversed by cysteine | Bos taurus | |
| Fe2+ | - |
Bos taurus | |
| Fe3+ | inhibition reversed by diphosphate | Bos taurus | |
| heparin | inhibition can be prevented by addition of NaCl | Bos taurus | |
| heparin | - |
Homo sapiens | |
| nitrated hyaluronic acid | - |
Bos taurus | |
| polymer from gentisic acid | - |
Homo sapiens | |
| quinol | irreversible inhibition | Homo sapiens | |
| quinone | irreversible inhibition | Homo sapiens | |
| rutin | - |
Bos taurus | |
| serum protein | inhibition strongly influenced by salts, that in- or decrease the effect | Bos taurus | |
| Zn2+ | - |
Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | secreted from cell culture | Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | more effective activation than with NaCl | Bos taurus | |
| diphosphate | 30% activation | Bos taurus | |
| MgCl2 | more effective activation than with NaCl | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hyaluronic acid + H2O | Cavia porcellus | - |
? | - |
? | |
| hyaluronic acid + H2O | Homo sapiens | - |
? | - |
? | |
| hyaluronic acid + H2O | Sus scrofa | - |
? | - |
? | |
| hyaluronic acid + H2O | Bos taurus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Cavia porcellus | - |
guinea pig | - |
| Homo sapiens | - |
- |
- |
| Ovis aries aries | - |
ovine | - |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Homo sapiens | - |
| semen | - |
Homo sapiens | - |
| semen | - |
Bos taurus | - |
| skin | - |
Sus scrofa | - |
| skin | - |
Bos taurus | - |
| synovia | - |
Homo sapiens | - |
| synovia | - |
Bos taurus | - |
| testis | - |
Bos taurus | - |
| testis | - |
Ovis aries aries | - |
| umbilical cord | - |
Homo sapiens | - |
| umbilical cord | - |
Sus scrofa | - |
| vitreous humor | - |
Homo sapiens | - |
| vitreous humor | - |
Sus scrofa | - |
| vitreous humor | - |
Bos taurus | - |
| vitreous humor | - |
Ovis aries aries | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chondroitin sulfate A + H2O | acting at the same rate as with hyaluronic acid | Bos taurus | oligosaccharides + ? | - |
? | |
| chondroitin sulfate C + H2O | acting at the same rate as with hyaluronic acid | Bos taurus | ? | - |
? | |
| hyaluronic acid + H2O | - |
Cavia porcellus | ? | - |
? | |
| hyaluronic acid + H2O | - |
Homo sapiens | ? | - |
? | |
| hyaluronic acid + H2O | - |
Sus scrofa | ? | - |
? | |
| hyaluronic acid + H2O | - |
Bos taurus | ? | - |
? | |
| hyaluronic acid + H2O | complete hydrolysis | Bos taurus | hyaluronic acid tetrasaccharides + ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
in the presence of gelatin twice as active as at 37°C | Bos taurus |
| 65 | - |
complete inactivation above | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
broad optimum below, pH-activity curve depends on hyaluronate-enzyme and enzyme-inhibitor interactions | Bos taurus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
