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Literature summary for 3.2.1.33 extracted from
- Cloning and characterization of glycogen-debranching enzyme from hyperthermophilic archaeon Sulfolobus shibatae (2007), J. Microbiol. Biotechnol., 17, 792-799.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain MC 1061 | Saccharolobus shibatae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DMSO | 80.8% residual activity at 10% (v/v) | Saccharolobus shibatae |  |
| ethanol | 83.3% residual activity at 10% (v/v) | Saccharolobus shibatae | |
| methanol | 91.6% residual activity at 10% (v/v) | Saccharolobus shibatae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12.23 | - |
6-O-alpha-maltosyl-beta-cyclodextrin | at 70°C in 50 mM sodium acetate buffer (pH 5.5) | Saccharolobus shibatae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 41.7% increase of activity at 5 mM | Saccharolobus shibatae | |
| Ca2+ | 27.3% increase of activity at 5 mM | Saccharolobus shibatae | |
| Co2+ | 15% increase of activity at 5 mM | Saccharolobus shibatae | |
| Fe2+ | 40.6% increase of activity at 5 mM | Saccharolobus shibatae | |
| Mg2+ | 17.2% increase of activity at 5 mM | Saccharolobus shibatae | |
| Mn2+ | 62.2% increase of activity at 5 mM | Saccharolobus shibatae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus shibatae | - |
strain ATCC 51178 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA affinity chromatography | Saccharolobus shibatae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-O-alpha-maltosyl-beta-cyclodextrin + H2O | - |
Saccharolobus shibatae | maltose + beta-cyclodextrin | - |
? | |
| amylopectin + H2O | 100% activity | Saccharolobus shibatae | amylopectin + D-glucose | - |
? | |
| amylose + H2O | 16.9% activity compared to amylopectin | Saccharolobus shibatae | ? | - |
? | |
| glycogen + H2O | 73.5% activity compared to amylopectin | Saccharolobus shibatae | glycogen + D-glucose | - |
? | |
| pullulan + H2O | 36.8% activity compared to amylopectin | Saccharolobus shibatae | ? | - |
? | |
| rice starch + H2O | 82.4% activity compared to amylopectin | Saccharolobus shibatae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| amylo-1,6-glucosidase | part of the putative glycogen-debranching enzyme SSGDE | Saccharolobus shibatae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Saccharolobus shibatae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
about 20% of the enzyme activity remains after 60 min of incubation at 100°C | Saccharolobus shibatae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Saccharolobus shibatae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6.5 | - |
Saccharolobus shibatae |
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