Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.33 extracted from

  • Becker, J.U.; Long, T.J.; Fischer, E.H.
    Purification and properties of debranching enzyme from dogfish muscle (1977), Biochemistry, 16, 291-297.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information no activating by usual sugar phosphates or nucleotide effectors of glycolysis such as AMP, ADP, ATP, uridine diphosphoglucose, glucose-1-phosphate, glucose-6-phosphate and phosphate Squalus acanthias

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid) 50% inhibition, reversed by glycogen Squalus acanthias
additional information no inhibition by usual sugar phosphates or nucleotide effectors of glycolysis such as AMP, ADP, ATP, uridine diphosphoglucose, glucose-1-phosphate, glucose-6-phosphate and phosphate Squalus acanthias
Tris 15 mM: 75% inhibition Squalus acanthias

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
glycogen debranching enzyme, PAGE and SDS-PAGE Squalus acanthias
162000
-
glycogen debranching enzyme, carboxymethylation prior to sedimentation equilibrium Squalus acanthias

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Squalus acanthias glycogen debranching enzyme might play an important role in the structure of glycogen particles, or the control of its metabolism ?
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol stable in ethanol precipitation with final concentration of 23% ethanol Squalus acanthias

Organism

Organism UniProt Comment Textmining
Squalus acanthias
-
pacific dogfish
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no modification no covalently bound phosphate or carbohydrate, method would not have detected less than 5% carbohydrate Squalus acanthias

Purification (Commentary)

Purification (Comment) Organism
glycogen debranching enzyme Squalus acanthias

Reaction

Reaction Comment Organism Reaction ID
6-O-alpha-D-glucosyl cyclomaltoheptaose + H2O = D-glucose + cyclomaltoheptaose regulation of enzyme activity Squalus acanthias

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
Squalus acanthias
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3
-
maleate buffer, pH 6.0, 19°C Squalus acanthias

Storage Stability

Storage Stability Organism
frozen, solution in Tris-glycerol buffer, 3 months, without loss of activity Squalus acanthias

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-(1-6)-glucosyl cyclohexaamylose + H2O
-
Squalus acanthias cyclohexaamylose + D-glucose
-
r
glycogen + H2O liver glycogen and shellfish glycogen: one-tenth of activity compared with glycogen phosphorylase limit dextrin as substrate Squalus acanthias glycogen + D-glucose
-
r
glycogen phosphorylase-limit dextrin + H2O
-
Squalus acanthias limit dextrin + D-glucose
-
r
additional information glycogen debranching enzyme might play an important role in the structure of glycogen particles, or the control of its metabolism Squalus acanthias ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 160000, glycogen debranching enzyme, carboxymethylation prior to SDS-PAGE Squalus acanthias

Synonyms

Synonyms Comment Organism
glycogen debranching system EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system Squalus acanthias

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
19
-
-
Squalus acanthias

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
unstable above Squalus acanthias
30
-
rapid inactivation Squalus acanthias

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6.3
-
Squalus acanthias

pH Range

pH Minimum pH Maximum Comment Organism
4.6 9 inactive below pH 4.6 and above pH 9 Squalus acanthias