Application | Comment | Organism |
---|---|---|
analysis | useful in structural determination of glycogen and amylopectin | Oryctolagus cuniculus |
medicine | diagnosis, even prenatal, of type III glycogen storage disease: marked decrease or absence of amylo-1,6-glucosidase, glycogen debranching enzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
additional information | - |
- |
Oryctolagus cuniculus |
70000 | - |
glycogen debranching enzyme, three basic subunits, 1 * 122000, 1 * 85000, 1 * 70000, carboxymethylation prior to SDS-PAGE and gel electrophoresis in the presence of urea | Saccharomyces cerevisiae |
85000 | - |
glycogen debranching enzyme, three basic subunits, 1 * 122000, 1 * 85000, 1 * 70000, carboxymethylation prior to SDS-PAGE and gel electrophoresis in the presence of urea | Saccharomyces cerevisiae |
122000 | - |
glycogen debranching enzyme, three basic subunits, 1 * 122000, 1 * 85000, 1 * 70000, carboxymethylation prior to SDS-PAGE and gel electrophoresis in the presence of urea | Saccharomyces cerevisiae |
210000 | - |
glycogen debranching enzyme, gel filtration and PAGE | Saccharomyces cerevisiae |
267000 | 279000 | glycogen debranching enzyme, equilibrium ultracentrifugation | Oryctolagus cuniculus |
280000 | - |
- |
Saccharomyces cerevisiae |
280000 | - |
glycogen debranching enzyme, sedimentation equilibrium | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycogen phosphorylase-limit dextrin + H2O | Mammalia | phi-dextrin | ? | - |
? | |
glycogen phosphorylase-limit dextrin + H2O | Saccharomyces cerevisiae | phi-dextrin | ? | - |
? | |
glycogen phosphorylase-limit dextrin + H2O | Oryctolagus cuniculus | phi-dextrin | ? | - |
? | |
glycogen phosphorylase-limit dextrin + H2O | Oryctolagus cuniculus | total degradation of glycogen is accomplished by the combined action of glycogen phosphorylase and glycogen debranching enzyme | ? | - |
? | |
additional information | Mammalia | glycogen debranching system may be regarded as an integral part of the overall phosphorolytic pathway for the degradation of glycogen | ? | - |
? | |
additional information | Saccharomyces cerevisiae | glycogen debranching system may be regarded as an integral part of the overall phosphorolytic pathway for the degradation of glycogen | ? | - |
? | |
additional information | Oryctolagus cuniculus | glycogen debranching system may be regarded as an integral part of the overall phosphorolytic pathway for the degradation of glycogen | ? | - |
? | |
additional information | Mammalia | glycogen debranching system, indirect debranching enzymes, is the dominant type of glycogen debranching mechanism, where glycogen is stored | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mammalia | - |
generally present in mammalian tissues | - |
Oryctolagus cuniculus | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae | - |
baker's yeast | - |
Purification (Comment) | Organism |
---|---|
glycogen debranching enzyme | Saccharomyces cerevisiae |
glycogen debranching enzyme | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Mammalia | - |
leukocyte | - |
Mammalia | - |
leukocyte | - |
Homo sapiens | - |
liver | - |
Mammalia | - |
liver | - |
Homo sapiens | - |
liver | - |
Oryctolagus cuniculus | - |
muscle | - |
Mammalia | - |
muscle | - |
Homo sapiens | - |
muscle | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4 | - |
substrate: glycogen phosphorylase-limit dextrin | Oryctolagus cuniculus |
6 | - |
- |
Saccharomyces cerevisiae |
7.6 | - |
- |
Oryctolagus cuniculus |
8.3 | - |
- |
Oryctolagus cuniculus |
8.4 | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 D-glucose | - |
Oryctolagus cuniculus | 6-O-alpha-D-glucosyl-D-glucose + H2O | isomaltose | r | |
63-alpha-glucosyl maltotetraose + H2O | branched pentasaccharide "fast B5" | Mammalia | maltotetraose + D-glucose | - |
r | |
63-alpha-glucosyl maltotetraose + H2O | branched pentasaccharide "fast B5" | Oryctolagus cuniculus | maltotetraose + D-glucose | - |
r | |
63-alpha-maltotriosyl maltotetraose + H2O | branched heptasaccharide B7 | Mammalia | ? | - |
? | |
63-alpha-maltotriosyl maltotetraose + H2O | branched heptasaccharide B7 | Oryctolagus cuniculus | ? | - |
? | |
alpha-(1-6)-glucosyl cyclohexaamylose + H2O | 6-alpha-glucosyl alpha-Schardinger dextrin, cyclodextrin | Saccharomyces cerevisiae | cyclohexaamylose + D-glucose | - |
r | |
alpha-(1-6)-glucosyl cyclohexaamylose + H2O | 6-alpha-glucosyl alpha-Schardinger dextrin, cyclodextrin | Mammalia | cyclohexaamylose + D-glucose | alpha-Schardinger dextrin | r | |
alpha-(1-6)-glucosyl cyclohexaamylose + H2O | 6-alpha-glucosyl alpha-Schardinger dextrin, cyclodextrin | Saccharomyces cerevisiae | cyclohexaamylose + D-glucose | alpha-Schardinger dextrin | r | |
alpha-(1-6)-glucosyl cyclohexaamylose + H2O | 6-alpha-glucosyl alpha-Schardinger dextrin, cyclodextrin | Oryctolagus cuniculus | cyclohexaamylose + D-glucose | alpha-Schardinger dextrin | r | |
alpha-dextrin + H2O | - |
Mammalia | alpha-dextrin + D-glucose | - |
? | |
alpha-dextrin + H2O | - |
Saccharomyces cerevisiae | alpha-dextrin + D-glucose | - |
? | |
amylopectin + H2O | very poor substrate | Oryctolagus cuniculus | amylopectin + D-glucose | partially debranched | ? | |
amylopectin + H2O | slowly hydrolyzed | Mammalia | amylopectin + D-glucose | - |
? | |
amylopectin + H2O | slowly hydrolyzed | Saccharomyces cerevisiae | amylopectin + D-glucose | - |
? | |
amylopectin + H2O | slowly hydrolyzed | Saccharomyces cerevisiae | amylopectin + D-glucose | partially debranched | ? | |
amylopectin + H2O | phi-dextrin, beta-dextrin and phi-beta-dextrin from amylopectin | Saccharomyces cerevisiae | amylopectin + D-glucose | partially debranched | ? | |
amylopectin + H2O | phi-dextrin, beta-dextrin and phi-beta-dextrin from amylopectin | Oryctolagus cuniculus | amylopectin + D-glucose | partially debranched | ? | |
amylose + H2O | - |
Saccharomyces cerevisiae | ? | - |
r | |
amylose + H2O | - |
Oryctolagus cuniculus | ? | - |
r | |
beta-amylase limit dextrin + H2O | beta-dextrin | Saccharomyces cerevisiae | limit dextrin + D-glucose | - |
r | |
beta-amylase limit dextrin + H2O | beta-dextrin | Oryctolagus cuniculus | limit dextrin + D-glucose | - |
r | |
D-glucose + maltooligosaccharide | - |
Saccharomyces cerevisiae | maltooligosaccharide | branched | r | |
D-glucose + maltooligosaccharide | - |
Oryctolagus cuniculus | maltooligosaccharide | branched | r | |
glycogen + H2O | rabbit liver glycogen: very poor substrate, shellfish glycogen: not a substrate | Oryctolagus cuniculus | glycogen + D-glucose | - |
r | |
glycogen + H2O | native glycogen from shellfish and rabbit liver | Saccharomyces cerevisiae | glycogen + D-glucose | - |
r | |
glycogen + H2O | reverse reaction: incorporation of glucose into glycogen | Mammalia | glycogen + D-glucose | - |
r | |
glycogen + H2O | reverse reaction: incorporation of glucose into glycogen | Homo sapiens | glycogen + D-glucose | - |
r | |
glycogen + H2O | reverse reaction: incorporation of glucose into glycogen | Saccharomyces cerevisiae | glycogen + D-glucose | - |
r | |
glycogen + H2O | reverse reaction: incorporation of glucose into glycogen | Oryctolagus cuniculus | glycogen + D-glucose | - |
r | |
glycogen + H2O | rabbit liver glycogen: slowly | Oryctolagus cuniculus | glycogen + D-glucose | - |
r | |
glycogen phosphorylase-limit dextrin + H2O | best substrate | Mammalia | limit dextrin + D-glucose | - |
r | |
glycogen phosphorylase-limit dextrin + H2O | best substrate | Saccharomyces cerevisiae | limit dextrin + D-glucose | - |
r | |
glycogen phosphorylase-limit dextrin + H2O | best substrate | Oryctolagus cuniculus | limit dextrin + D-glucose | partially debranched glycogen | r | |
glycogen phosphorylase-limit dextrin + H2O | phi-dextrin | Mammalia | limit dextrin + D-glucose | - |
r | |
glycogen phosphorylase-limit dextrin + H2O | phi-dextrin | Saccharomyces cerevisiae | limit dextrin + D-glucose | - |
r | |
glycogen phosphorylase-limit dextrin + H2O | phi-dextrin | Oryctolagus cuniculus | limit dextrin + D-glucose | partially debranched glycogen | r | |
glycogen phosphorylase-limit dextrin + H2O | phi-dextrin | Mammalia | ? | - |
? | |
glycogen phosphorylase-limit dextrin + H2O | phi-dextrin | Saccharomyces cerevisiae | ? | - |
? | |
glycogen phosphorylase-limit dextrin + H2O | phi-dextrin | Oryctolagus cuniculus | ? | - |
? | |
glycogen phosphorylase-limit dextrin + H2O | total degradation of glycogen is accomplished by the combined action of glycogen phosphorylase and glycogen debranching enzyme | Oryctolagus cuniculus | ? | - |
? | |
additional information | substrate specificities | Mammalia | ? | - |
? | |
additional information | substrate specificities | Saccharomyces cerevisiae | ? | - |
? | |
additional information | substrate specificities | Oryctolagus cuniculus | ? | - |
? | |
additional information | 63-alpha-glucosyl maltotriose: not a substrate | Oryctolagus cuniculus | ? | - |
? | |
additional information | panose: not a substrate | Saccharomyces cerevisiae | ? | - |
? | |
additional information | pullulan: not a substrate | Mammalia | ? | - |
? | |
additional information | glycogen debranching system may be regarded as an integral part of the overall phosphorolytic pathway for the degradation of glycogen | Mammalia | ? | - |
? | |
additional information | glycogen debranching system may be regarded as an integral part of the overall phosphorolytic pathway for the degradation of glycogen | Saccharomyces cerevisiae | ? | - |
? | |
additional information | glycogen debranching system may be regarded as an integral part of the overall phosphorolytic pathway for the degradation of glycogen | Oryctolagus cuniculus | ? | - |
? | |
additional information | glycogen debranching system, indirect debranching enzymes, is the dominant type of glycogen debranching mechanism, where glycogen is stored | Mammalia | ? | - |
? | |
phosphorylase beta-amylase limit dextrin + H2O | limit dextrin, beta-phi-dextrin, derived from the action of beta-amylase on the phosphorylase limit dextrin | Saccharomyces cerevisiae | limit dextrin + D-glucose | - |
r | |
phosphorylase beta-amylase limit dextrin + H2O | limit dextrin, beta-phi-dextrin, derived from the action of beta-amylase on the phosphorylase limit dextrin | Oryctolagus cuniculus | limit dextrin + D-glucose | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | evidence for a subunit of 120000 | Oryctolagus cuniculus |
trimer | - |
Saccharomyces cerevisiae |
trimer | glycogen debranching enzyme, three basic subunits, 1 * 122000, 1 * 85000, 1 * 70000, carboxymethylation prior to SDS-PAGE and gel electrophoresis in the presence of urea | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
glycogen debranching system | EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system | Mammalia |
glycogen debranching system | EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system | Homo sapiens |
glycogen debranching system | EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system | Saccharomyces cerevisiae |
glycogen debranching system | EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH-optimum depends on type of buffer | Oryctolagus cuniculus |
additional information | - |
pH-optimum depends on substrate and type of buffer, overview | Oryctolagus cuniculus |
6 | 6.6 | - |
Saccharomyces cerevisiae |
6.6 | - |
anionic buffer, substrate: glycogen phosphorylase limit dextrin | Oryctolagus cuniculus |
7.2 | - |
cationic buffer, substrate: glycogen phosphorylase limit dextrin | Oryctolagus cuniculus |