Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Aspergillus niger |
expression in Pichia pastoris | Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0304 | - |
4-nitrophenyl beta-D-glucoside | - |
Aspergillus niger | |
0.0383 | - |
4-nitrophenyl beta-D-glucoside | - |
Neurospora crassa | |
0.378 | - |
GlcAbeta(1-6)Gal | - |
Neurospora crassa | |
0.422 | - |
GlcAbeta(1-6)Gal | - |
Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56682 | - |
x * 56682, calculated, mature protein, x * 85700, SDS-PAGE | Aspergillus niger |
58123 | - |
x * 58123, calculated, mature protein, x * 90100, SDS-PAGE | Neurospora crassa |
85700 | - |
x * 56682, calculated, mature protein, x * 85700, SDS-PAGE | Aspergillus niger |
90100 | - |
x * 58123, calculated, mature protein, x * 90100, SDS-PAGE | Neurospora crassa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Neurospora crassa | Q7SFB0 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | sequence contains 12 putative glycosylation sites | Neurospora crassa |
glycoprotein | sequence contains 12 putative glycosylation sites. Treatment with endoglycanase F reduces the molecular mass to 55000 Da | Aspergillus niger |
proteolytic modification | sequence contains a putative signal sequence, calculated molecular mass of immature protein is 58859 | Aspergillus niger |
proteolytic modification | sequence contains a putative signal sequence, calculated molecular mass of immature protein is 60235 | Neurospora crassa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
29.3 | - |
pH 6.0, 37°C | Neurospora crassa |
30.6 | - |
pH 4.0, 37°C | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methyl-beta-GlcA(1-6)beta-Gal(1-6)Gal + H2O | - |
Neurospora crassa | 4-methyl-D-glucuronate + beta-Gal(1-6)Gal | 4.8% of the activity with 4-nitrophenyl beta-D-glucuronic acid | ? | |
4-methyl-beta-GlcA(1-6)beta-Gal(1-6)Gal + H2O | - |
Aspergillus niger | 4-methyl-D-glucuronate + beta-Gal(1-6)Gal | 42.6% of the activity with 4-nitrophenyl beta-D-glucuronic acid | ? | |
4-nitrophenyl beta-D-glucoside + H2O | - |
Aspergillus niger | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucoside + H2O | - |
Neurospora crassa | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucuronic acid + H2O | - |
Aspergillus niger | 4-nitrophenol + D-glucuronic acid | - |
? | |
4-nitrophenyl beta-D-glucuronic acid + H2O | - |
Neurospora crassa | 4-nitrophenol + D-glucuronic acid | - |
? | |
beta-GlcA(1-6)beta-Gal(1-6)Gal + H2O | - |
Neurospora crassa | D-glucuronate + beta-Gal(1-6)Gal | 67.4% of the activity with 4-nitrophenyl beta-D-glucuronic acid | ? | |
beta-GlcA(1-6)betaGal(1-6)Gal + H2O | - |
Aspergillus niger | D-glucuronate + beta-Gal(1-6)Gal | 94.6% of the activity with 4-nitrophenyl beta-D-glucuronic acid | ? | |
GlcA-beta(1-6)Gal + H2O | 49.9% of the activity with 4-nitrophenyl beta-D-glucuronic acid | Neurospora crassa | D-glucuronate + D-galactopyranose | - |
? | |
GlcAbeta(1-6)Gal + H2O | 85.7% of the activity with 4-nitrophenyl beta-D-glucuronic acid | Aspergillus niger | D-glucuronate + D-galactopyranose | - |
? | |
additional information | enzyme additionally catalyzes the transglycosylation of glucuronic acid residues from 4-nitrophenyl beta-D-glucuronic acid to various monosaccharide acceptors such as glucose, galactose, and xylose | Aspergillus niger | ? | - |
? | |
additional information | enzyme additionally catalyzes the transglycosylation of glucuronic acid residues from 4-nitrophenyl beta-D-glucuronic acid to various monosaccharide acceptors such as glucose, galactose, and xylose | Neurospora crassa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56682, calculated, mature protein, x * 85700, SDS-PAGE | Aspergillus niger |
? | x * 58123, calculated, mature protein, x * 90100, SDS-PAGE | Neurospora crassa |
Synonyms | Comment | Organism |
---|---|---|
GlcAase | - |
Aspergillus niger |
GlcAase | - |
Neurospora crassa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Aspergillus niger |
45 | - |
- |
Neurospora crassa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
30 min, 50% loss of activity | Aspergillus niger |
50 | - |
30 min, 50% loss of activity | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | 4 | - |
Aspergillus niger |
6 | - |
activity gradually decreases below | Neurospora crassa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1 | 7 | stable | Aspergillus niger |
6 | 9 | stable | Neurospora crassa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus niger | calculated | - |
5.1 |
Neurospora crassa | calculated | - |
6.8 |