Literature summary for 3.2.1.31 extracted from

Xiong, A.S.; Peng, R.H.; Zhuang, J.; Liu, J.G.; Xu, F.; Cai, B.; Guo, Z.K.; Qiao, Y.S.; Chen, J.M.; Zhang, Z.; Yao, Q.H.
Directed evolution of beta-galactosidase from Escherichia coli into beta-glucuronidase (2007), J. Biochem. Mol. Biol., 40, 419-425.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli DH5alpha cells	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
L527I/M543I	158% increased activity compared to the wild type enzyme	Escherichia coli
S193N/G466A/Q951R	212% increased activity compared to the wild type enzyme	Escherichia coli
S193N/T266A/Q267R/Q626R	167% increased activity compared to the wild type enzyme	Escherichia coli
S193N/V411A/D448G	172% increased activity compared to the wild type enzyme	Escherichia coli
T266A/Q267R/Q626R	137% increased activity compared to the wild type enzyme	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel chelate affinity chromatography	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
o-nitrophenyl-beta-D-glucuronide + H2O	-	Escherichia coli	o-nitrophenol + D-glucuronic acid	-	?
p-nitrophenyl-beta-D-glucuronide + H2O	-	Escherichia coli	p-nitrophenol + D-glucuronic acid	-	?

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Release 2023.1 (January 2023)