Literature summary for 3.2.1.31 extracted from

Gehrmann, M.C.; Opper, M.; Sedlacek, H.H.; Bosslet, K.; Czech, J.
Biochemical properties of recombinant human beta-glucuronidase synthesized in baby hamster kidney cells (1994), Biochem. J., 301, 821-828.

Cloned(Commentary)

Cloned (Comment)	Organism
expression vectors pMCG-GLUC and pMCG-dGLUC containing cDNA coding for human beta-glucuronidase and a C-terminal-truncated form of beta-glucuronidase respectively are transfected into BHK cells. The stable clones B702 expressing wild-type and B688 expressing mutant beta-glucuronidase. In the mutant enzyme a stop codon is introduced after the CAC/His-637 codon, thereby eliminating 14 amino acids from the C-terminus	Homo sapiens

Cloned (Comment)

Organism

expression vectors pMCG-GLUC and pMCG-dGLUC containing cDNA coding for human beta-glucuronidase and a C-terminal-truncated form of beta-glucuronidase respectively are transfected into BHK cells. The stable clones B702 expressing wild-type and B688 expressing mutant beta-glucuronidase. In the mutant enzyme a stop codon is introduced after the CAC/His-637 codon, thereby eliminating 14 amino acids from the C-terminus

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	in the mutant enzyme a stop codon is introduced after the CAC/His-637 codon, thereby eliminating 14 amino acids from the C-terminus. Thereby the last Cys at position 644 is eliminated. In the mutant, covalent linkage between two monomers is no longer observed, indicating that Cys44 is involved in intermolecular disulfide-bond formation	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.3	-	4-methylumbelliferyl-beta-D-glucuronide	pH 5, 37°C	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	wild-type enzyme	Homo sapiens	-	-
intracellular	recombinant enzyme in BHK cells	Homo sapiens	5622	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
79000	-	2 * 79000, intracellular human beta-glucuronidase in transfected BHK cells, SDS-PAGE	Homo sapiens
83000	-	4 * 83000, the enzyme consists of two disulfide-linked dimers, wild-type enzyme and secreted recombinant enzyme, SDS-PAGE	Homo sapiens
390000	-	wild-type enzyme, gel filtration	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Homo sapiens
proteolytic modification	human beta-glucuronidase remains intracellular in BHK cells after synthesis undergoes a proteolytic processing event, i.e. a reduction in mass of 3000-4000 Da	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
31.33	-	-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl-beta-D-glucuronide + H2O	-	Homo sapiens	4-methylumbelliferone + D-glucuronic acid	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 79000, intracellular human beta-glucuronidase in transfected BHK cells, SDS-PAGE	Homo sapiens
tetramer	4 * 83000, the enzyme consists of two disulfide-linked dimers, wild-type enzyme and secreted recombinant enzyme, SDS-PAGE	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
68	-	pH 6, stable for up to 3 h	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4	-	-	Homo sapiens