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Literature summary for 3.2.1.31 extracted from
- beta-Glucuronidase of rat preputial gland. Crystallization, properties, carbohydrate composition, and subunits (1975), J. Biochem., 77, 427-438.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
General Stability
| General Stability | Organism |
|---|---|
| bovine serum albumin, stabilization | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | 2.9 | p-nitrophenyl-beta-D-glucuronide | - |
Rattus norvegicus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 250000 | 310000 | sedimentation equilibrium centrifugation | Rattus norvegicus |
| 250000 | 310000 | gel electrophoresis | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | carbohydrate composition | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| preputial gland | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucuronide + H2O | - |
Rattus norvegicus | 4-nitrophenol + D-glucuronic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | SDS-PAGE | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
more stable in neutral buffers | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
or 7.8, stable at, depending on buffer | Rattus norvegicus |
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