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Literature summary for 3.2.1.3 extracted from

  • Li, Z.; Ji, K.; Dong, W.; Ye, X.; Wu, J.; Zhou, J.; Wang, F.; Chen, Q.; Fu, L.; Li, S.; Huang, Y.; Cui, Z.
    Cloning, heterologous expression, and enzymatic characterization of a novel glucoamylase GlucaM from Corallococcus sp. strain EGB (2017), Protein Expr. Purif., 129, 122-127 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Corallococcus coralloides

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 18% activation at 1 mM, 25% inhibition at 5 mM Corallococcus coralloides
Co2+ complete inhibition at 1-5 mM Corallococcus coralloides
Cr3+ 62% inhibition at 1 mM, complete inhibition at 5 mM Corallococcus coralloides
Cu2+ 22% inhibition at 1 mM, complete inhibition at 5 mM Corallococcus coralloides
EDTA 22% inhibition at 10 mM Corallococcus coralloides
Fe2+ 27% inhibition at 1 mM, complete inhibition at 5 mM Corallococcus coralloides
Fe3+ 62% inhibition at 1 mM, complete inhibition at 5 mM Corallococcus coralloides
Mn2+ 34.5% inhibition at 1-5 mM Corallococcus coralloides
Ni2+ 44% inhibition at 1 mM, 70% at 5 mM Corallococcus coralloides
SDS 30% inhibition at 5 mg/ml Corallococcus coralloides
Triton X-100 20% inhibition at 20 mg/ml Corallococcus coralloides
Zn2+ 28% inhibition at 1 mM, 43% at 5 mM Corallococcus coralloides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Corallococcus coralloides

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 18% activation at 1 mM, 25% inhibition at 5 mM Corallococcus coralloides
Ca2+ 8.5% activation at 1 mM, 5.5% at 5 mM Corallococcus coralloides
K+ 34% activation at 1 mM, 31% at 5 mM Corallococcus coralloides
Mg2+ 50% activation at 1 mM, 8% at 5 mM Corallococcus coralloides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Corallococcus coralloides
-
?
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
additional information low concentrations (10%) of methanol, ethanol, acetonitrile, and DMSO do not affect the enzyme's activity Corallococcus coralloides

Organism

Organism UniProt Comment Textmining
Corallococcus coralloides A0A0H3WKG6
-
-
Corallococcus coralloides CCTCC M2012528 A0A0H3WKG6
-
-
Corallococcus coralloides EGB A0A0H3WKG6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme 50.2fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Corallococcus coralloides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
180
-
purified recombinant enzyme, pH 7.0, 50°C Corallococcus coralloides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-cyclodextrin + H2O
-
Corallococcus coralloides ?
-
?
amylopectin + H2O from potato Corallococcus coralloides ?
-
?
amylose + H2O from potato Corallococcus coralloides ?
-
?
amylose + H2O from potato Corallococcus coralloides CCTCC M2012528 ?
-
?
amylose + H2O from potato Corallococcus coralloides EGB ?
-
?
dextran + H2O
-
Corallococcus coralloides ?
-
?
dextran + H2O
-
Corallococcus coralloides CCTCC M2012528 ?
-
?
dextran + H2O
-
Corallococcus coralloides EGB ?
-
?
dextrin + H2O
-
Corallococcus coralloides ?
-
?
dextrin + H2O
-
Corallococcus coralloides CCTCC M2012528 ?
-
?
dextrin + H2O
-
Corallococcus coralloides EGB ?
-
?
glycogen + H2O
-
Corallococcus coralloides ?
-
?
additional information substrate specificity, overview Corallococcus coralloides ?
-
?
additional information substrate specificity, overview Corallococcus coralloides CCTCC M2012528 ?
-
?
additional information substrate specificity, overview Corallococcus coralloides EGB ?
-
?
pullulan + H2O
-
Corallococcus coralloides ?
-
?
pullulan + H2O
-
Corallococcus coralloides CCTCC M2012528 ?
-
?
pullulan + H2O
-
Corallococcus coralloides EGB ?
-
?
starch + H2O
-
Corallococcus coralloides ?
-
?
starch + H2O soluble starch and raw starch from sweet potato and corn Corallococcus coralloides ?
-
?

Subunits

Subunits Comment Organism
? x * 72920, about, sequence calculation Corallococcus coralloides

Synonyms

Synonyms Comment Organism
GHF15 glucoamylase
-
Corallococcus coralloides
GlucaM
-
Corallococcus coralloides
glucoamylase
-
Corallococcus coralloides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Corallococcus coralloides

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 recombinant enzyme, activity range, profile overview Corallococcus coralloides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 80 recombinant enzyme GlucaM retains full activity after 1 h of incubation at below 60°C and retains about 60% of the original activity after 1 h at 70°C, but it loses 60% of the original activity after 1 h of incubation at 80°C Corallococcus coralloides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Corallococcus coralloides

pH Range

pH Minimum pH Maximum Comment Organism
3 10 recombinant enzyme, activity range, profile overview Corallococcus coralloides

pI Value

Organism Comment pI Value Maximum pI Value
Corallococcus coralloides sequence calculation
-
6.31