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Literature summary for 3.2.1.3 extracted from

  • Carrasco, M.; Alcaino, J.; Cifuentes, V.; Baeza, M.
    Purification and characterization of a novel cold adapted fungal glucoamylase (2017), Microb. Cell Fact., 16, 75 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.3

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination, analysis and comparisons Tetracladium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information 4.5 g/l with soluble starch Tetracladium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Tetracladium sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme does not depend on Ca2+ for its hydrolytic activity Tetracladium sp.

Organism

Organism UniProt Comment Textmining
Tetracladium sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O soluble starch Tetracladium sp. ?
-
 ?

Subunits

Subunits Comment Organism
More enzyme structure modelling and comparison of the structural model of Tetracladium sp. glucoamylase with the solved structure of the Hypocrea jecorina glucoamylase Tetracladium sp.

Synonyms

Synonyms Comment Organism
glucoamylase
-
 Tetracladium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Tetracladium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 glucoamylase secreted by Tetracladium sp. is a novel cold-adapted enzyme Tetracladium sp.
4 60 at 22°C and 4°C, the enzyme shows 55% and 25% of its maximal activity, lower activity above 45°C, highest activity between 30°C and 45°C Tetracladium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22 30 purified enzyme, pH 6.0, 6-8 h, stable Tetracladium sp.
40
-
 purified enzyme, pH 6.0, loss of 30% activity after 2 h, loss of 60% activity after 8 h Tetracladium sp.
50
-
 purified enzyme, pH 6.0, 1 h, loss of 90% activity Tetracladium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.75
-
 starch soluble starch, pH 6.0, 30°C Tetracladium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Tetracladium sp.

General Information

General Information Comment Organism
additional information enzyme structure modelling and comparison of the structural model of Tetracladium sp. glucoamylase with the solved structure of the Hypocrea jecorina glucoamylase Tetracladium sp.