Any feedback?
Literature summary for 3.2.1.3 extracted from
- Purification and biochemical characterization of extracellular glucoamylase from Paenibacillus amylolyticus strain (2019), J. Basic Microbiol., 59, 375-384 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 12% activation at 1 mM, 20% at 10 mM | Paenibacillus amylolyticus |  |
| DTT | 12% activation at 10 mM | Paenibacillus amylolyticus | |
Application
| Application | Comment | Organism |
|---|---|---|
| biofuel production | the secreted glucoamylase from Paenibacillus amylolyticus strain NEO03 possesses properties suitable for saccharification processes such as biofuel production | Paenibacillus amylolyticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Paenibacillus amylolyticus | |
| Ca2+ | - |
Paenibacillus amylolyticus | |
| Cu2+ | - |
Paenibacillus amylolyticus | |
| Fe2+ | - |
Paenibacillus amylolyticus | |
| Fe3+ | - |
Paenibacillus amylolyticus | |
| Hg2+ | - |
Paenibacillus amylolyticus | |
| K+ | - |
Paenibacillus amylolyticus | |
| additional information | no inhibition by neuraminidase inhibitor and EDTA at 1-10 mM | Paenibacillus amylolyticus | |
| N-bromosuccimide | 40% inhibition at 10 mM, 54% at 1 mM | Paenibacillus amylolyticus | |
| N-ethylmaleimide | - |
Paenibacillus amylolyticus | |
| Na+ | - |
Paenibacillus amylolyticus | |
| PMSF | 53% inhibition at 10 mM | Paenibacillus amylolyticus | |
| SDS | 60% inhibition at 1 mM, 80% at 10 mM | Paenibacillus amylolyticus | |
| tosylphenylalanylchloromethyl ketone | 10% inhibition at 1 mM, 20% at 10 mM | Paenibacillus amylolyticus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic values Km and Vmax with soluble starch are 2.84 mg/ml and 239.2 U/ml, respectively, Lineweaver-Burk kinetics | Paenibacillus amylolyticus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Paenibacillus amylolyticus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | activates | Paenibacillus amylolyticus | |
| additional information | no dependence on Ca2+ ions | Paenibacillus amylolyticus | |
| Zn2+ | activates | Paenibacillus amylolyticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
native extracellular enzyme, gel filtration | Paenibacillus amylolyticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Paenibacillus amylolyticus | glucoamylase hydrolyzes alpha-1,4- and alpha-1,6-glycosidic linkages from the non-reducing ends of starch molecule, and specifically forms D-glucose units | ? | - |
? | |
| additional information | Paenibacillus amylolyticus NEO03 | glucoamylase hydrolyzes alpha-1,4- and alpha-1,6-glycosidic linkages from the non-reducing ends of starch molecule, and specifically forms D-glucose units | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenibacillus amylolyticus | - |
- |
- |
| Paenibacillus amylolyticus NEO03 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme 2.73fold from submerged culture by starch adsorption chromatography and gel filtration | Paenibacillus amylolyticus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | submerged fermentation conditions | Paenibacillus amylolyticus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2300.5 | - |
purified extracellular enzyme, pH 7.0, 40°C | Paenibacillus amylolyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Paenibacillus amylolyticus | D-glucose + ? | - |
? | |
| amylopectin + H2O | - |
Paenibacillus amylolyticus NEO03 | D-glucose + ? | - |
? | |
| amylose + H2O | - |
Paenibacillus amylolyticus | D-glucose + ? | - |
? | |
| amylose + H2O | - |
Paenibacillus amylolyticus NEO03 | D-glucose + ? | - |
? | |
| maltose + H2O | - |
Paenibacillus amylolyticus | 2 D-glucose | - |
? | |
| maltose + H2O | - |
Paenibacillus amylolyticus NEO03 | 2 D-glucose | - |
? | |
| additional information | glucoamylase hydrolyzes alpha-1,4- and alpha-1,6-glycosidic linkages from the non-reducing ends of starch molecule, and specifically forms D-glucose units | Paenibacillus amylolyticus | ? | - |
? | |
| additional information | glucoamylase hydrolyzes alpha-1,4- and alpha-1,6-glycosidic linkages from the non-reducing ends of starch molecule, and specifically forms D-glucose units | Paenibacillus amylolyticus NEO03 | ? | - |
? | |
| starch + H2O | the enzyme preferentially hydrolyzes all the starch substrates, soluble and raw. High substrate specificity is demonstrated towards soluble starch | Paenibacillus amylolyticus | D-glucose + ? | - |
? | |
| starch + H2O | the enzyme preferentially hydrolyzes all the starch substrates, soluble and raw. High substrate specificity is demonstrated towards soluble starch | Paenibacillus amylolyticus NEO03 | D-glucose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 66000, native extracellular enzyme, SDS-PAGE | Paenibacillus amylolyticus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucoamylase | - |
Paenibacillus amylolyticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Paenibacillus amylolyticus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
80 | 30% of maximal activity at 0°C, 80% at 60°C, 70% at 20°C and 80°C, maximal activity at 40°C, profile overview | Paenibacillus amylolyticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | 6 h, pH 7.0, completely stable at 30°C, loss of 10% activity at 40°C, loss of 20% at 50°C, and of 30% at 60°C | Paenibacillus amylolyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Paenibacillus amylolyticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 13 | over 20% of maximal activity within this range, profile overview | Paenibacillus amylolyticus |
| 3.5 | 10 | over 50% of maximal activity within this range, profile overview | Paenibacillus amylolyticus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | 6 h, 40°C, loss of 10% activity at pH 6.0, loss of 20% at pH 7.0, and of 40% at pH 8.0 | Paenibacillus amylolyticus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
