Cloned (Comment) | Organism |
---|---|
recombinant expression of the glucoamylase subunit Ct-MGAM splice form N20 and the sucrase subunit Ct-SI in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(+)-catechin | noncompetitive inhibition | Mus musculus | |
caffeic acid | noncompetitive inhibition | Mus musculus | |
chlorogenic acid | noncompetitive inhibition | Mus musculus | |
epigallocatechin gallate | EGCG, noncompetitive inhibition | Mus musculus | |
gallic acid | noncompetitive inhibition | Mus musculus | |
additional information | kinetics and mechanism of inhibition of the recombinant glucoamylase subunit Ct-MGAM and sucrase subunit Ct-SI, overview | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Mus musculus | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Mus musculus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q6XK24 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant glucoamylase subunit Ct-MGAM splice form N20 and subunit Ct-SI from Spodoptera frugiperda Sf9 cells | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | - |
Mus musculus | ? | - |
? | |
starch + H2O | soluble starch | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glucoamylase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00151 | - |
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-SI | Mus musculus | epigallocatechin gallate | |
0.0017 | - |
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-MGAM | Mus musculus | epigallocatechin gallate | |
0.0018 | - |
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-SI | Mus musculus | chlorogenic acid | |
0.003 | - |
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-MGAM | Mus musculus | chlorogenic acid | |
0.0204 | - |
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-MGAM | Mus musculus | (+)-catechin | |
0.0216 | - |
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-SI | Mus musculus | (+)-catechin | |
0.0249 | - |
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-MGAM | Mus musculus | gallic acid | |
0.0313 | - |
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-SI | Mus musculus | gallic acid | |
0.0565 | - |
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-MGAM | Mus musculus | caffeic acid | |
0.0582 | - |
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-SI | Mus musculus | caffeic acid |