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Literature summary for 3.2.1.3 extracted from

  • Simsek, M.; Quezada-Calvillo, R.; Ferruzzi, M.G.; Nichols, B.L.; Hamaker, B.R.
    Dietary phenolic compounds selectively inhibit the individual subunits of maltase-glucoamylase and sucrase-isomaltase with the potential of modulating glucose release (2015), J. Agric. Food Chem., 63, 3873-3879 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of the glucoamylase subunit Ct-MGAM splice form N20 and the sucrase subunit Ct-SI in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
(+)-catechin noncompetitive inhibition Mus musculus
caffeic acid noncompetitive inhibition Mus musculus
chlorogenic acid noncompetitive inhibition Mus musculus
epigallocatechin gallate EGCG, noncompetitive inhibition Mus musculus
gallic acid noncompetitive inhibition Mus musculus
additional information kinetics and mechanism of inhibition of the recombinant glucoamylase subunit Ct-MGAM and sucrase subunit Ct-SI, overview Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Mus musculus 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Mus musculus
-
?
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus Q6XK24
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant glucoamylase subunit Ct-MGAM splice form N20 and subunit Ct-SI from Spodoptera frugiperda Sf9 cells Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O
-
Mus musculus ?
-
?
starch + H2O soluble starch Mus musculus ?
-
?

Synonyms

Synonyms Comment Organism
glucoamylase
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00151
-
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-SI Mus musculus epigallocatechin gallate
0.0017
-
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-MGAM Mus musculus epigallocatechin gallate
0.0018
-
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-SI Mus musculus chlorogenic acid
0.003
-
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-MGAM Mus musculus chlorogenic acid
0.0204
-
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-MGAM Mus musculus (+)-catechin
0.0216
-
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-SI Mus musculus (+)-catechin
0.0249
-
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-MGAM Mus musculus gallic acid
0.0313
-
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-SI Mus musculus gallic acid
0.0565
-
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-MGAM Mus musculus caffeic acid
0.0582
-
pH not specified in the publication, 37°C, recombinant glucoamylase subunit Ct-SI Mus musculus caffeic acid