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Literature summary for 3.2.1.3 extracted from

  • Suyama, Y.; Muraki, N.; Kusunoki, M.; Miyake, H.
    Crystal structure of the starch-binding domain of glucoamylase from Aspergillus niger (2017), Acta Crystallogr. Sect. F, 73, 550-554 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of the starch-binding domain (SBD)-containing polypeptide (amino acids 509-616) of glucoamylase in Escherichia coli Aspergillus niger

Crystallization (Commentary)

Crystallization (Comment) Organism
purified starch-binding domain (SBD)-containing polypeptide (amino acids 509-616) of Aspergillus niger glucoamylase, hanging drop vapour diffusion method, using 2.0-2.3 M ammonium sulfate, 1.7% w/v PEG 400, 15% v/v glycerol, and 85 mM HEPES, pH 7.5, as the reservoir solution, the asymmetric unit of the starch-binding domain is composed of four protein molecules, 20°C, two weeks, X-ray diffraction structure determination and analysis at 2.0 A resolution, NMR structures of the SBD of Aspergillus niger glucoamylase (PDB entries 1kum, 1kul, 1aco, and 1acz) are used as search models for molecular replacement Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Aspergillus niger
-
?
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger P69328
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O
-
Aspergillus niger ?
-
?

Subunits

Subunits Comment Organism
More the starch-binding domain (SBD) of glucoamylase from Aspergillus niger is a small globular protein containing a disulfide bond. The structure of the SBD has been determined by NMR. Cys509 and Cys604 are at the N- and C-termini, respectively, and they are linked by a disulfide bond, analysis of the conformation surrounding the disulfide bond, overview Aspergillus niger

Synonyms

Synonyms Comment Organism
GlaA
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Aspergillus niger
glucoamylase
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Aspergillus niger