Literature summary for 3.2.1.3 extracted from

Sauer, J.; Abou Hachem, M.; Svensson, B.; Jensen, K.J.; Thygesen, M.B.
Kinetic analysis of inhibition of glucoamylase and active site mutants via chemoselective oxime immobilization of acarbose on SPR chip surfaces (2013), Carbohydr. Res., 375, 21-28.

Search for more literature for 3.2.1.3

Application

Application	Comment	Organism
analysis	method for anchoring native acarbose to gold chip surfaces for surface plasmon resonance studies employing glucoamylase as analyte. The key method is the chemoselective and protecting group-free oxime functionalization of the pseudo-tetrasaccharide-based inhibitor acarbose. At pH 7.0 the association and dissociation rate constants for the acarbose-glucoamylase interaction are 10000 per M and s and 103 per s, respectively, and the conformational change to a tight enzyme-inhibitor complex affects the dissociation rate constant by a factor of 100 per s. The acarbose-presenting surface plason resonance surfaces can be used as a glucoamylase sensor that allows rapid, label-free affinity screening of small carbohydrate-based inhibitors in solution	Aspergillus niger

Protein Variants

Protein Variants	Comment	Organism
E180Q	active site mutant. For inhibitor acarbose, a rapid binding event is apparently intersected by a slower secondary binding event. Mutant shows a dramatically higher Kd value for acarbose	Aspergillus niger
R54L	active site mutant. For inhibitor acarbose, a rapid binding event is apparently intersected by a slower secondary binding event. Mutant shows a dramatically higher Kd value for acarbose	Aspergillus niger
Y175F	mutation in subsite +3. Mutant displays only minor differences to wild-type in affinities to inhibitors acarbose and an acarbose conjugate	Aspergillus niger

Inhibitors

Inhibitors	Comment	Organism	Structure
1-deoxynojirimycin	-	Aspergillus niger
acarbose	-	Aspergillus niger

Organism

Organism	UniProt	Comment	Textmining
Aspergillus niger	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	-	Aspergillus niger	-

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Release 2023.1 (January 2023)