Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a series of hybrid enzymes by interchanging domains of glucoamylase Sta1 from Saccharomyces cerevisiae and beta-glucosidase Bgl1 from Saccharomycopsis fibuligera strain ATCC 9947 based on the homology-based structural models of the two proteins. The replacement of native Bgl1 signal peptide by that of Sta1, SPS-Bgl1, increases the production of the enzyme by about threefold without affecting the ratio between the values of activity associated to cells and free in the medium | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
var. diastaticus | - |
Saccharomyces cerevisiae BY4741 | - |
var. diastaticus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
soluble starch + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
soluble starch + H2O | - |
Saccharomyces cerevisiae BY4741 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | glucoamylase is a two-domain protein composed by a N-terminal serinethreonine-rich domain and a C-terminal domain with the typical structure of the catalytic domain of fungal glucoamylases | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
glucoamylase | - |
Saccharomyces cerevisiae |
Sta1 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
additional information | the replacement of native beta-glucosidase Bgl1 signal peptide by that of Sta1, SPS-Bgl1, increases the production of the enzyme by about threefold without affecting the ratio between the values of activity associated to cells and free in the medium | Saccharomyces cerevisiae |