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Literature summary for 3.2.1.3 extracted from
- An extracellular glucoamylase produced by endophytic fungus EF6 (2011), Appl. Biochem. Microbiol., 47, 412-418.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| DMSO | - |
endophytic fungus EF6 |  |
| DTT | - |
endophytic fungus EF6 | |
| EDTA | - |
endophytic fungus EF6 | |
| glycerol | - |
endophytic fungus EF6 | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | - |
endophytic fungus EF6 | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
endophytic fungus EF6 | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | endophytic fungus EF6 | |
| Co2+ | activates | endophytic fungus EF6 | |
| Mg2+ | activates | endophytic fungus EF6 | |
| Mn2+ | activates | endophytic fungus EF6 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
gel filtration | endophytic fungus EF6 |
| 62031 | - |
1 * 62200, SDS-PAGE, 1 * 62031, mass spectrometry | endophytic fungus EF6 |
| 62200 | - |
1 * 62200, SDS-PAGE, 1 * 62031, mass spectrometry | endophytic fungus EF6 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| endophytic fungus EF6 | - |
isolated from Thai medicinal plants | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 14.49fold from culture medium by ammonium sulfate fractionation, anion exchange chromatography and gel filtration | endophytic fungus EF6 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 758 | - |
purified enzyme, substrate is soluble starch, pH 6.0, 50°C | endophytic fungus EF6 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | soluble starch is the best substrate, various raw starches, i.e. soluble starch, corn, tapioca, wheat, rice, sticky rice starch, are used as substrates | endophytic fungus EF6 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 62200, SDS-PAGE, 1 * 62031, mass spectrometry | endophytic fungus EF6 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucoamylase | - |
endophytic fungus EF6 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
endophytic fungus EF6 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 90 | activity range, profile, overview | endophytic fungus EF6 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
purified enzyme, 1 h, stable up to, rapid decrease in activity above | endophytic fungus EF6 |
| 55 | - |
purified enzyme, 1 h, 45% activity remaining | endophytic fungus EF6 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
endophytic fungus EF6 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | activity range, profile, overview | endophytic fungus EF6 |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
purified enzyme, 1 h, 20% activity remaining | endophytic fungus EF6 |
| 4 | 7 | purified enzyme, 1 h, 50°C, stable | endophytic fungus EF6 |
| 9 | - |
purified enzyme, 1 h, 40% activity remaining | endophytic fungus EF6 |
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