Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant S54P/T314A/H415Y enzymes in Saccharomyces cerevisiae | Aspergillus awamori |
Protein Variants | Comment | Organism |
---|---|---|
S54P/T314A/H415Y | the mutant enzyme is more thermostable compared to the wild-type enzyme at 70°C. The mutation does not affect the protein secretion nor the production of the enzyme | Aspergillus awamori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
mutant S54P/T314A/H415Y | Aspergillus awamori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus awamori | - |
- |
- |
Aspergillus awamori C468 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme mutant S54P/T314A/H415Y from Saccharomyces cerevisiae by acarbose affinity chromatography | Aspergillus awamori |
Synonyms | Comment | Organism |
---|---|---|
glucoamylase | - |
Aspergillus awamori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
mutant S54P/T314A/H415Y | Aspergillus awamori |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified recombinant enzyme mutant S54P/T314A/H415Y, stable up to, irreversible thermo-inactivation of the mutant enzyme follows first-order kinetics | Aspergillus awamori |
65 | - |
purified recombinant enzyme mutant S54P/T314A/H415Y, half-life is 76 min | Aspergillus awamori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
mutant S54P/T314A/H415Y | Aspergillus awamori |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | the recombinant enzyme mutant S54P/T314A/H415Y retains 80% of its activity within this range | Aspergillus awamori |