Cloned (Comment) | Organism |
---|---|
genes amyC and amyD, expression in Pichia pastoris strain X33 | Rhizopus arrhizus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
maltotriose | substrate inhibition of AmyC | Rhizopus arrhizus | |
additional information | AmyC, but not AmyD, exhibits substrate inhibition, the Ki for substrate inhibition decreases with increasing length of the oligosaccharides. AmyC accumulates an enzyme maltose-maltotriose dead-end complex in the steady state, kinetics and modelling, overview | Rhizopus arrhizus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state and pre-steady-state kinetic measurements using maltooligosacchrides as substrates, analysis and modelling, overview | Rhizopus arrhizus | |
0.224 | - |
maltopentaose | recombinant AmyC, pH 5.5, 40°C | Rhizopus arrhizus | |
0.276 | - |
maltotetraose | recombinant AmyC, pH 5.5, 40°C | Rhizopus arrhizus | |
0.287 | - |
maltohexaose | recombinant AmyC, pH 5.5, 40°C | Rhizopus arrhizus | |
0.487 | - |
maltotriose | recombinant AmyC, pH 5.5, 40°C | Rhizopus arrhizus | |
0.53 | - |
maltose | recombinant AmyC, pH 5.5, 40°C | Rhizopus arrhizus | |
0.62 | - |
maltohexaose | recombinant AmyD, pH 5.5, 40°C | Rhizopus arrhizus | |
0.93 | - |
maltopentaose | recombinant AmyD, pH 5.5, 40°C | Rhizopus arrhizus | |
1.51 | - |
maltotetraose | recombinant AmyD, pH 5.5, 40°C | Rhizopus arrhizus | |
1.88 | - |
maltotriose | recombinant AmyD, pH 5.5, 40°C | Rhizopus arrhizus | |
3.78 | - |
maltose | recombinant AmyD, pH 5.5, 40°C | Rhizopus arrhizus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizopus arrhizus | - |
two enzymes AmyC and AmyD, genes amyC or amyD | - |
Rhizopus arrhizus 99-880 | - |
two enzymes AmyC and AmyD, genes amyC or amyD | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Rhizopus arrhizus |
Purification (Comment) | Organism |
---|---|
recombinant AmyC and AmyD from Pichia pastoris strain X33 culture supernatant by affinity chromatography, the recombinant enzymes are deglycosylated with endoglycosidase H and PNGase F | Rhizopus arrhizus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltohexaose + H2O | - |
Rhizopus arrhizus | maltopentaose + D-glucose | - |
? | |
maltohexaose + H2O | - |
Rhizopus arrhizus 99-880 | maltopentaose + D-glucose | - |
? | |
maltopentaose + H2O | - |
Rhizopus arrhizus | maltotetraose + beta-D-glucose | - |
? | |
maltopentaose + H2O | - |
Rhizopus arrhizus 99-880 | maltotetraose + beta-D-glucose | - |
? | |
maltose + H2O | - |
Rhizopus arrhizus | beta-D-glucose + D-glucose | - |
? | |
maltose + H2O | - |
Rhizopus arrhizus 99-880 | beta-D-glucose + D-glucose | - |
? | |
maltotetraose + H2O | - |
Rhizopus arrhizus | maltotriose + beta-D-glucose | - |
? | |
maltotetraose + H2O | - |
Rhizopus arrhizus 99-880 | maltotriose + beta-D-glucose | - |
? | |
maltotriose + 2 H2O | - |
Rhizopus arrhizus | 3 beta-D-glucose | - |
? | |
maltotriose + 2 H2O | - |
Rhizopus arrhizus 99-880 | 3 beta-D-glucose | - |
? | |
soluble starch + H2O | - |
Rhizopus arrhizus | beta-D-glucose + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-1,4-glucan glucohydrolase | - |
Rhizopus arrhizus |
AmyC | - |
Rhizopus arrhizus |
AmyD | - |
Rhizopus arrhizus |
amyloglucosidase | - |
Rhizopus arrhizus |
glucoamylase | - |
Rhizopus arrhizus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
AmyC | Rhizopus arrhizus |
50 | - |
AmyD | Rhizopus arrhizus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
30 min, purified recombinant AmyC and AmyD, completely stable, rapid inactivation above | Rhizopus arrhizus |
50 | - |
30 min, inactivation of both purified recombinant AmyC and AmyD | Rhizopus arrhizus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.08 | - |
maltose | recombinant AmyD, pH 5.5, 40°C | Rhizopus arrhizus | |
9.3 | - |
maltose | recombinant AmyC, pH 5.5, 40°C | Rhizopus arrhizus | |
19.5 | - |
maltotriose | recombinant AmyD, pH 5.5, 40°C | Rhizopus arrhizus | |
35.1 | - |
soluble starch | recombinant AmyD, pH 5.5, 40°C | Rhizopus arrhizus | |
38.4 | - |
maltohexaose | recombinant AmyD, pH 5.5, 40°C | Rhizopus arrhizus | |
40 | - |
maltotetraose | recombinant AmyD, pH 5.5, 40°C | Rhizopus arrhizus | |
43.8 | - |
maltopentaose | recombinant AmyD, pH 5.5, 40°C | Rhizopus arrhizus | |
54.7 | - |
maltotriose | recombinant AmyC, pH 5.5, 40°C | Rhizopus arrhizus | |
58.2 | - |
soluble starch | recombinant AmyC, pH 5.5, 40°C | Rhizopus arrhizus | |
71.1 | - |
maltotetraose | recombinant AmyC, pH 5.5, 40°C | Rhizopus arrhizus | |
71.3 | - |
maltohexaose | recombinant AmyC, pH 5.5, 40°C | Rhizopus arrhizus | |
73.7 | - |
maltopentaose | recombinant AmyC, pH 5.5, 40°C | Rhizopus arrhizus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
AmyC, pH profile, overview | Rhizopus arrhizus |
6 | - |
AmyD, pH profile, overview | Rhizopus arrhizus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | 30 min, purified recombinant AmyC, 70% of maximal activity at pH 3.0, 85% at pH 8.0, profile overview | Rhizopus arrhizus |
3.5 | 8 | 30 min, purified recombinant AmyD, 70% of maximal activity at pH 3.5, 85% at pH 8.0, inactive at pH 3.0, profile overview | Rhizopus arrhizus |
General Information | Comment | Organism |
---|---|---|
physiological function | glucoamylase enzymes catalyze hydrolysis of alpha(1-4) glycosidic bonds to release D-glucose residues from the non-reducing ends of starch and oligosaccharides. Glucoamylase also has limited ability to release D-glucose residues by promoting hydrolysis of alpha(1-6) linkages of amylopectin | Rhizopus arrhizus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.61 | - |
maltose | recombinant AmyD, pH 5.5, 40°C | Rhizopus arrhizus | |
10.4 | - |
maltotriose | recombinant AmyD, pH 5.5, 40°C | Rhizopus arrhizus | |
17.5 | - |
maltose | recombinant AmyC, pH 5.5, 40°C | Rhizopus arrhizus | |
26.5 | - |
maltotetraose | recombinant AmyD, pH 5.5, 40°C | Rhizopus arrhizus | |
46.9 | - |
maltopentaose | recombinant AmyD, pH 5.5, 40°C | Rhizopus arrhizus | |
61.9 | - |
maltohexaose | recombinant AmyD, pH 5.5, 40°C | Rhizopus arrhizus | |
112 | - |
maltotriose | recombinant AmyC, pH 5.5, 40°C | Rhizopus arrhizus | |
248 | - |
maltohexaose | recombinant AmyC, pH 5.5, 40°C | Rhizopus arrhizus | |
257 | - |
maltotetraose | recombinant AmyC, pH 5.5, 40°C | Rhizopus arrhizus | |
329 | - |
maltopentaose | recombinant AmyC, pH 5.5, 40°C | Rhizopus arrhizus |