Application | Comment | Organism |
---|---|---|
synthesis | entrapment of amyloglucosidase into dipalmitoylphosphatidylcholine multilamellar vesicles and large unilamellar vesicles for biocatalysis inside liposomes and bioanalytical applications, overview | Aspergillus niger |
Protein Variants | Comment | Organism |
---|---|---|
additional information | entrapment of amyloglucosidase into dipalmitoylphosphatidylcholine multilamellar vesicles and large unilamellar vesicles, vesicle formation, method optimization, enzyme activity is very stable during the first three batch runs, overview | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | product inhibition, kinetics | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of starch hydrolysis by AMG modeled for two different systems, free enzyme in aqueous solution and entrapped enzyme within vesicles in aqueous suspension, overview, Michaelis-Menten kinetic model with product inhibition for intrinsic kinetics | Aspergillus niger | |
1.15 | - |
starch | pH 4.5, 55°C, multilamellar liposome-entrapped enzyme | Aspergillus niger | |
1.55 | - |
starch | pH 4.5, 55°C, free soluble enzyme | Aspergillus niger | |
1.64 | - |
starch | pH 4.5, 55°C, large unilamellar liposome-entrapped enzyme | Aspergillus niger |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | liposome | Aspergillus niger | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Aspergillus niger | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Aspergillus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.76 | 8.86 | liposome-entrapped enzyme | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | - |
Aspergillus niger | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
amyloglucosidase | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 5.0, soluble enzyme half-life: 160 h, liposome-entrapped enzyme shows full activity after 180 h | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
assay at | Aspergillus niger |