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Literature summary for 3.2.1.3 extracted from
- Effect of aniline coupling on kinetic and thermodynamic properties of Fusarium solani glucoamylase (2007), Appl. Microbiol. Biotechnol., 73, 1290-1298.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | purified glucoamylase is chemically modified by cross-linking with aniline hydrochloride in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide for 1, 7, or 13 min, resulting in aniline-coupled glucoamylase-1/ACG-1, aniline-coupled glucoamylase-7/ACG-7, and 13 min aniline-coupled glucoamylase-13/ACG-13, the aniline coupling of GA has profound enhancing effects on temperature, pH optima, and pKas of active site residues, overview | Fusarium solani |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of native enzyme, and aniline-coupled glucoamylase-1/ACG-1, aniline-coupled glucoamylase-7/ACG-7, and 13 min aniline-coupled glucoamylase-13/ACG-13, overview | Fusarium solani | |
| 1.4 | - |
starch | pH 4.5, 40°C, ACG-7 | Fusarium solani |  |
| 1.6 | - |
starch | pH 4.5, 40°C, native enzyme | Fusarium solani | |
| 1.7 | - |
starch | pH 4.5, 40°C, ACG-13 | Fusarium solani | |
| 2 | - |
starch | pH 4.5, 40°C, ACG-1 | Fusarium solani | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium solani | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activities of native enzyme, and aniline-coupled glucoamylase-1/ACG-1, aniline-coupled glucoamylase-7/ACG-7, and 13 min aniline-coupled glucoamylase-13/ACG-13, overview | Fusarium solani |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | - |
Fusarium solani | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucoamylase | - |
Fusarium solani |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
assay at, native enzyme | Fusarium solani |
| 55 | - |
assay at, aniline-coupled glucoamylases | Fusarium solani |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
native enzyme shows a half-life of 128.37 min | Fusarium solani |
| 60 | - |
native enzyme shows a half-life of 25.67 min, ACG-13 shows a half-life of 67.3 min | Fusarium solani |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 217 | - |
starch | pH 4.5, 40°C, native enzyme | Fusarium solani | |
| 354 | - |
starch | pH 4.5, 40°C, ACG-13 | Fusarium solani | |
| 367 | - |
starch | pH 4.5, 40°C, ACG-7 | Fusarium solani | |
| 487 | - |
starch | pH 4.5, 40°C, ACG-1 | Fusarium solani | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
native enzyme and aniline-coupled glucoamylase ACG-1 | Fusarium solani |
| 5.5 | - |
aniline-coupled glucoamylases ACG-7 and ACG-13 | Fusarium solani |
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