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Literature summary for 3.2.1.3 extracted from

  • Wong, D.W.; Batt, S.B.; Lee, C.C.; Wagschal, K.; Robertson, G.H.
    Characterization of active Lentinula edodes glucoamylase expressed and secreted by Saccharomyces cerevisiae (2005), Protein J., 24, 455-463.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae, secretion of functional enzyme to the medium from recombinant cells Lentinula edodes

Inhibitors

Inhibitors Comment Organism Structure
D-galactose 22% inhibition, recombinant enzyme Lentinula edodes
D-glucosamine 68% inhibition, recombinant enzyme Lentinula edodes
D-glucose 44% inhibition, recombinant enzyme Lentinula edodes
D-xylose 64% inhibition, recombinant enzyme Lentinula edodes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3
-
soluble starch pH 5.3, 40°C, recombinant enzyme Lentinula edodes

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 45% activation the recombinant enzyme Lentinula edodes
additional information no effect on the recombinant enzyme by Li+, Zn2+, Mg2+, Cu+, Ca2+, and EDTA Lentinula edodes

Organism

Organism UniProt Comment Textmining
Lentinula edodes
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 2.4% carbohydrate content, recombinant enzyme Lentinula edodes
proteolytic modification cleavage of signal peptide of the recombinant enzyme expressed in Saccharomyces cerevisiae Lentinula edodes

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme to homogeneity from Saccharomyces cerevisiae cell culture medium, by ammonium sulfate fractionation, and anion exchange and affinity chromatography Lentinula edodes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O preferred substrate Lentinula edodes amylopectin + beta-D-glucose
-
?
amylose + H2O low activity Lentinula edodes amylose + beta-D-glucose
-
?
soluble starch + H2O
-
Lentinula edodes beta-D-glucose + ?
-
?
soluble starch + H2O
-
Lentinula edodes beta-D-glucose + soluble starch
-
?
starch + H2O 16times and 29times higher activity with soluble starch compared to wheat or corn starch granules, respectively Lentinula edodes starch + beta-D-glucose
-
?

Synonyms

Synonyms Comment Organism
GLA
-
Lentinula edodes
glucoamylase
-
Lentinula edodes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
recombinant enzyme Lentinula edodes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable up to, recombinant enzyme Lentinula edodes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4833
-
soluble starch pH 5.3, 40°C, recombinant enzyme Lentinula edodes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6
-
recombinant enzyme Lentinula edodes

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.4 6.4 stability range, recombinant enzyme Lentinula edodes