Literature summary for 3.2.1.3 extracted from

Ichikawa, K.; Tonozuka, T.; Mizuno, M.; Tanabe, Y.; Kamitori, S.; Nishikawa, A.; Sakano, Y.
Crystallization and preliminary X-ray analysis of Thermoactinomyces vulgaris R-47 maltooligosaccharide-metabolizing enzyme homologous to glucoamylase (2005), Acta Crystallogr. Sect. F, 61, 302-304.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, hanging drop vapour diffusion method, 20°C, 0.0015 ml of 20 mg/ml enzyme solution is mixed with 0.0015 ml of reservoir solution containing 10% w/v PEG 5000 monomethyl ether, 186 mM D-glucose, 10 mM DTT, and 1.0 M lithium chloride, in 100 mM MES, pH 6.1, cryoprotection by 20% w/v PEG 6000, 20% v/v 2-methyl-2,4-pentanediol, 2.5 mM CaCl2, 40 mM MES, pH 6.1, X-ray diffraction structure determination and analysis at 3.3 A resolution	Thermoactinomyces vulgaris

Crystallization (Comment)

Organism

purified recombinant enzyme, hanging drop vapour diffusion method, 20°C, 0.0015 ml of 20 mg/ml enzyme solution is mixed with 0.0015 ml of reservoir solution containing 10% w/v PEG 5000 monomethyl ether, 186 mM D-glucose, 10 mM DTT, and 1.0 M lithium chloride, in 100 mM MES, pH 6.1, cryoprotection by 20% w/v PEG 6000, 20% v/v 2-methyl-2,4-pentanediol, 2.5 mM CaCl2, 40 mM MES, pH 6.1, X-ray diffraction structure determination and analysis at 3.3 A resolution

Thermoactinomyces vulgaris

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
155000	-	gel filtration	Thermoactinomyces vulgaris

Organism

Organism	UniProt	Comment	Textmining
Thermoactinomyces vulgaris	-	-	-
Thermoactinomyces vulgaris R-47	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli strain MV1184 by heat treatment at 56°C for 30 min, two steps of ion exchange chromatography, and gel filtration	Thermoactinomyces vulgaris

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
maltooligosaccharides + H2O	high activity	Thermoactinomyces vulgaris	maltooligosaccharides + beta-D-glucose	-	?
maltooligosaccharides + H2O	high activity	Thermoactinomyces vulgaris R-47	maltooligosaccharides + beta-D-glucose	-	?
starch + H2O	soluble starch, very low activity	Thermoactinomyces vulgaris	starch + beta-D-glucose	-	?
starch + H2O	soluble starch, very low activity	Thermoactinomyces vulgaris R-47	starch + beta-D-glucose	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Thermoactinomyces vulgaris

Synonyms

Synonyms	Comment	Organism
GA	-	Thermoactinomyces vulgaris
glucoamylase	-	Thermoactinomyces vulgaris
maltooligosaccharide-metabolizing enzyme	-	Thermoactinomyces vulgaris
tGA	-	Thermoactinomyces vulgaris

pI Value

Organism	Comment	pI Value Maximum	pI Value
Thermoactinomyces vulgaris	isoelectric focusing	-	5.7