Crystallization (Comment) | Organism |
---|---|
tGA crystals are grown at 24°C by the hanging-drop vapor diffusion method, 0.002 ml of a 14 mg/ml tGA solution in 20 mM Tris-HCl, pH 7.5, are mixed with the same volume of reservoier solution containing 14-16% polyethylene glycol 3350, 100 mm Tris-HCl, pH 8.0 and 200 mM Li2SO4, crystals of native tGA and tGA complexed with acarbose diffract to 2.2 A and 2.1 A resolution, respectively | Thermoanaerobacterium thermosaccharolyticum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acarbose | strong inhibition | Thermoanaerobacterium thermosaccharolyticum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
gel filtration | Thermoanaerobacterium thermosaccharolyticum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Thermoanaerobacterium thermosaccharolyticum | - |
beta-D-glucose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacterium thermosaccharolyticum | O85672 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermoanaerobacterium thermosaccharolyticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | - |
Thermoanaerobacterium thermosaccharolyticum | beta-D-glucose + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tGA | - |
Thermoanaerobacterium thermosaccharolyticum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000000001 | - |
acarbose | - |
Thermoanaerobacterium thermosaccharolyticum |