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Literature summary for 3.2.1.3 extracted from

  • Aleshin, A.E.; Feng, P.H.; Honzatko, R.B.; Reilly, P.J.
    Crystal structure and evolution of a prokaryotic glucoamylase (2003), J. Mol. Biol., 327, 61-73.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
tGA crystals are grown at 24°C by the hanging-drop vapor diffusion method, 0.002 ml of a 14 mg/ml tGA solution in 20 mM Tris-HCl, pH 7.5, are mixed with the same volume of reservoier solution containing 14-16% polyethylene glycol 3350, 100 mm Tris-HCl, pH 8.0 and 200 mM Li2SO4, crystals of native tGA and tGA complexed with acarbose diffract to 2.2 A and 2.1 A resolution, respectively Thermoanaerobacterium thermosaccharolyticum

Inhibitors

Inhibitors Comment Organism Structure
acarbose strong inhibition Thermoanaerobacterium thermosaccharolyticum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
gel filtration Thermoanaerobacterium thermosaccharolyticum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Thermoanaerobacterium thermosaccharolyticum
-
beta-D-glucose + ?
-
?

Organism

Organism UniProt Comment Textmining
Thermoanaerobacterium thermosaccharolyticum O85672
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermoanaerobacterium thermosaccharolyticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O
-
Thermoanaerobacterium thermosaccharolyticum beta-D-glucose + ?
-
?

Synonyms

Synonyms Comment Organism
tGA
-
Thermoanaerobacterium thermosaccharolyticum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000000001
-
acarbose
-
Thermoanaerobacterium thermosaccharolyticum