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Literature summary for 3.2.1.3 extracted from

  • Sierks, M.R.; Svennson, B.
    Protein engineering of the relative specificity of glucoamylase from Aspergillus awamori based on sequence similarities between starch degrading enzymes (1994), Protein Eng., 7, 1479-1484.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
G183K slight increase in activity as compared with the wild-type enzyme towards maltose. The mutation broadens the optimal pH range for activity towards acidic as well as alkaline conditions. Selectivity of the mutant for alpha-1,4-linked disaccharides over alpha-1,6-linked disaccharides is enhanced 2.3fold to 3.5fold Aspergillus awamori
S119Y slight increase in activity as compared with the wild-type enzyme towards maltose. Selectivity of the mutant for alpha-1,4-linked disaccharides over alpha-1,6-linked disaccharides is enhanced 2.3fold to 3.5fold Aspergillus awamori
S184H slight increase in activity as compared with the wild-type enzyme towards maltose. The mutation broadens the optimal pH range for activity towards acidic as well as alkaline conditions. Selectivity of the mutant for alpha-1,4-linked disaccharides over alpha-1,6-linked disaccharides is enhanced 2.3fold to 3.5fold Aspergillus awamori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
maltoheptaose wild-type enzyme or mutant enzymes Gly183Lys or S184H Aspergillus awamori
0.158
-
maltoheptaose mutant enzyme S119Y Aspergillus awamori
0.17
-
maltopentaose mutant enzyme S119Y Aspergillus awamori
0.206
-
maltohexaose mutant enzyme S119Y Aspergillus awamori
0.25
-
maltotetraose mutant enzyme S119Y Aspergillus awamori
0.39
-
maltotriose mutant enzyme S119Y Aspergillus awamori
0.9
-
maltose mutant enzyme S184H Aspergillus awamori
1.08
-
maltose mutant enzyme G183K Aspergillus awamori
1.1
-
maltose mutant enzyme S119Y Aspergillus awamori
1.4
-
maltose wild-type enzyme Aspergillus awamori
17.4
-
isomaltose mutant enzyme S184H Aspergillus awamori
24.6
-
isomaltose wild-type enzyme Aspergillus awamori
39
-
isomaltose mutant enzyme Gly183Lys Aspergillus awamori
42
-
isomaltose mutant enzyme S119Y Aspergillus awamori

Organism

Organism UniProt Comment Textmining
Aspergillus awamori
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isomaltose + H2O
-
Aspergillus awamori glucose
-
?
maltoheptaose + H2O
-
Aspergillus awamori ?
-
?
maltohexaose + H2O
-
Aspergillus awamori ?
-
?
maltopentaose + H2O
-
Aspergillus awamori ?
-
?
maltose + H2O
-
Aspergillus awamori 2 glucose
-
?
maltotetraose + H2O
-
Aspergillus awamori ?
-
?
maltotriose + H2O
-
Aspergillus awamori maltose + glucose
-
?
starch + H2O 300fold preference for the alpha-1,4-glucosidic linkage over the alpha-1,6-glucosidic linkage Aspergillus awamori glucose + ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.29
-
isomaltose mutant enzyme S184H Aspergillus awamori
0.36
-
isomaltose mutant enzyme S119Y Aspergillus awamori
0.41
-
isomaltose wild-type enzyme Aspergillus awamori
0.53
-
isomaltose mutant enzyme G183K Aspergillus awamori
9.2
-
maltose wild-type enzyme Aspergillus awamori
9.8
-
maltose mutant S184H Aspergillus awamori
10.1
-
maltose mutant S119Yr Aspergillus awamori
10.4
-
maltose mutant G83K Aspergillus awamori
41.8
-
maltopentaose mutant enzyme S119Y Aspergillus awamori
45
-
maltotriose mutant enzyme S119Y Aspergillus awamori
49.9
-
maltotetraose mutant enzyme S119Y Aspergillus awamori
50.3
-
maltohexaose mutant enzyme S119Y Aspergillus awamori
57
-
maltoheptaose mutant enzyme S119Y Aspergillus awamori
66
-
maltoheptaose wild-type enzyme Aspergillus awamori
72
-
maltoheptaose mutant enzyme G183K Aspergillus awamori
79
-
maltoheptaose mutant enzyme S184H Aspergillus awamori