Protein Variants | Comment | Organism |
---|---|---|
G183K | slight increase in activity as compared with the wild-type enzyme towards maltose. The mutation broadens the optimal pH range for activity towards acidic as well as alkaline conditions. Selectivity of the mutant for alpha-1,4-linked disaccharides over alpha-1,6-linked disaccharides is enhanced 2.3fold to 3.5fold | Aspergillus awamori |
S119Y | slight increase in activity as compared with the wild-type enzyme towards maltose. Selectivity of the mutant for alpha-1,4-linked disaccharides over alpha-1,6-linked disaccharides is enhanced 2.3fold to 3.5fold | Aspergillus awamori |
S184H | slight increase in activity as compared with the wild-type enzyme towards maltose. The mutation broadens the optimal pH range for activity towards acidic as well as alkaline conditions. Selectivity of the mutant for alpha-1,4-linked disaccharides over alpha-1,6-linked disaccharides is enhanced 2.3fold to 3.5fold | Aspergillus awamori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
maltoheptaose | wild-type enzyme or mutant enzymes Gly183Lys or S184H | Aspergillus awamori | |
0.158 | - |
maltoheptaose | mutant enzyme S119Y | Aspergillus awamori | |
0.17 | - |
maltopentaose | mutant enzyme S119Y | Aspergillus awamori | |
0.206 | - |
maltohexaose | mutant enzyme S119Y | Aspergillus awamori | |
0.25 | - |
maltotetraose | mutant enzyme S119Y | Aspergillus awamori | |
0.39 | - |
maltotriose | mutant enzyme S119Y | Aspergillus awamori | |
0.9 | - |
maltose | mutant enzyme S184H | Aspergillus awamori | |
1.08 | - |
maltose | mutant enzyme G183K | Aspergillus awamori | |
1.1 | - |
maltose | mutant enzyme S119Y | Aspergillus awamori | |
1.4 | - |
maltose | wild-type enzyme | Aspergillus awamori | |
17.4 | - |
isomaltose | mutant enzyme S184H | Aspergillus awamori | |
24.6 | - |
isomaltose | wild-type enzyme | Aspergillus awamori | |
39 | - |
isomaltose | mutant enzyme Gly183Lys | Aspergillus awamori | |
42 | - |
isomaltose | mutant enzyme S119Y | Aspergillus awamori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus awamori | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isomaltose + H2O | - |
Aspergillus awamori | glucose | - |
? | |
maltoheptaose + H2O | - |
Aspergillus awamori | ? | - |
? | |
maltohexaose + H2O | - |
Aspergillus awamori | ? | - |
? | |
maltopentaose + H2O | - |
Aspergillus awamori | ? | - |
? | |
maltose + H2O | - |
Aspergillus awamori | 2 glucose | - |
? | |
maltotetraose + H2O | - |
Aspergillus awamori | ? | - |
? | |
maltotriose + H2O | - |
Aspergillus awamori | maltose + glucose | - |
? | |
starch + H2O | 300fold preference for the alpha-1,4-glucosidic linkage over the alpha-1,6-glucosidic linkage | Aspergillus awamori | glucose + ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
isomaltose | mutant enzyme S184H | Aspergillus awamori | |
0.36 | - |
isomaltose | mutant enzyme S119Y | Aspergillus awamori | |
0.41 | - |
isomaltose | wild-type enzyme | Aspergillus awamori | |
0.53 | - |
isomaltose | mutant enzyme G183K | Aspergillus awamori | |
9.2 | - |
maltose | wild-type enzyme | Aspergillus awamori | |
9.8 | - |
maltose | mutant S184H | Aspergillus awamori | |
10.1 | - |
maltose | mutant S119Yr | Aspergillus awamori | |
10.4 | - |
maltose | mutant G83K | Aspergillus awamori | |
41.8 | - |
maltopentaose | mutant enzyme S119Y | Aspergillus awamori | |
45 | - |
maltotriose | mutant enzyme S119Y | Aspergillus awamori | |
49.9 | - |
maltotetraose | mutant enzyme S119Y | Aspergillus awamori | |
50.3 | - |
maltohexaose | mutant enzyme S119Y | Aspergillus awamori | |
57 | - |
maltoheptaose | mutant enzyme S119Y | Aspergillus awamori | |
66 | - |
maltoheptaose | wild-type enzyme | Aspergillus awamori | |
72 | - |
maltoheptaose | mutant enzyme G183K | Aspergillus awamori | |
79 | - |
maltoheptaose | mutant enzyme S184H | Aspergillus awamori |