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Literature summary for 3.2.1.3 extracted from
- Purification and characterization of two extracellular glucoamylase isozymes from Lipomyces kononenkoae (1995), J. Biochem. Mol. Biol., 28, 375-381.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 6.57 mg/ml for glucoamylase I with starch as substrate, 4.52 mg/ml for glucoamylase II with starch as substrate. 0.15 mg/ml for glucoamylase II with alpha-cyclodextrin as substrate, 2.0 mg/ml for glucoamylase II with beta-cyclodextrin as substrate | Lipomyces kononenkoae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Lipomyces kononenkoae | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 128000 | - |
1 * 128000, glucoamylase II, SDS-PAGE | Lipomyces kononenkoae |
| 140000 | - |
glucoamylase II, gel filtration | Lipomyces kononenkoae |
| 150000 | - |
2 * 150000, glucoamylase I, SDS-PAGE | Lipomyces kononenkoae |
| 230000 | - |
glucoamylase I, gel filtration | Lipomyces kononenkoae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lipomyces kononenkoae | - |
CBS 5608 mutant | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | glucoamylase I contains 7.5% carbohydrate, glucoamylase II contains 6.5% carbohydrate | Lipomyces kononenkoae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glucoamylase I and II | Lipomyces kononenkoae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-cyclodextrin + H2O | - |
Lipomyces kononenkoae | glucose + ? | - |
? |  |
| beta-cyclodextrin + H2O | - |
Lipomyces kononenkoae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 150000, glucoamylase I, SDS-PAGE | Lipomyces kononenkoae |
| monomer | 1 * 128000, glucoamylase II, SDS-PAGE | Lipomyces kononenkoae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Lipomyces kononenkoae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | stable | Lipomyces kononenkoae |
| 70 | - |
rapid inactivation above | Lipomyces kononenkoae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Lipomyces kononenkoae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6.5 | stable | Lipomyces kononenkoae |
| 5 | 8 | stable | Lipomyces kononenkoae |
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Release 2023.1 (January 2023)
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