Literature summary for 3.2.1.28 extracted from

Lucio-Eterovic, A.K.; Jorge, J.A.; Polizeli, M.d.e. .L.; Terenzi, H.F.
Biochemical characterisation of the trehalase of thermophilic fungi an enzyme with mixed properties of neutral and acid trehalase (2005), Biochim. Biophys. Acta, 1723, 201-207 .

Activating Compound

Activating Compound	Comment	Organism	Structure
Ca2+	at pH 4.5, the enzyme is neither activated by calcium nor inhibited by EDTA or ATP. At pH 6.5, the enzyme is activated by calcium and inhibited by ATP	Mycothermus thermophilus
Ca2+	at pH 4.5, the enzyme is neither activated by calcium nor inhibited by EDTA or ATP. At pH 6.5, the enzyme is activated by calcium and inhibited by ATP	Trichocladium griseum

Inhibitors

Inhibitors	Comment	Organism
ATP	at pH 4.5, the enzyme is neither activated by calcium nor inhibited by EDTA or ATP. At pH 6.5, the enzyme is activated by calcium and inhibited by ATP	Mycothermus thermophilus
ATP	at pH 4.5, the enzyme is neither activated by calcium nor inhibited by EDTA or ATP. At pH 6.5, the enzyme is activated by calcium and inhibited by ATP	Trichocladium griseum
EDTA	at pH 4.5, the enzyme is neither activated by calcium nor inhibited by EDTA or ATP. At pH 6.5, the enzyme is activated by calcium and inhibited by ATP	Mycothermus thermophilus
EDTA	at pH 4.5, the enzyme is neither activated by calcium nor inhibited by EDTA or ATP. At pH 6.5, the enzyme is activated by calcium and inhibited by ATP	Trichocladium griseum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Mycothermus thermophilus	-	-
intracellular	-	Mycothermus thermophilus	5622	-
intracellular	-	Trichocladium griseum	5622	-

Organism

Organism	UniProt	Comment	Textmining
Mycothermus thermophilus	-	-	-
Trichocladium griseum	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.2	-	40°C, pH 4.5, extracellular isoform, activity resistant to EDTA inhibition	Mycothermus thermophilus
4.8	-	40°C, pH 4.5, intracellular isoform, activity resistant to EDTA inhibition	Mycothermus thermophilus
5.2	-	40°C, pH 4.5, extracellular isoform, activity sensitive to EDTA inhibition	Mycothermus thermophilus
7.7	-	40°C, pH 4.5, extracellular isoform, activity resistant to EDTA inhibition	Trichocladium griseum
7.7	-	40°C, pH 4.5, extracellular isoform, activity sensitive to EDTA inhibition	Trichocladium griseum
10.8	-	40°C, pH 4.5, intracellular isoform, activity sensitive to EDTA inhibition	Mycothermus thermophilus
11.2	-	40°C, pH 6.5, intracellular isoform, activity resistant to EDTA inhibition	Mycothermus thermophilus
11.5	-	40°C, pH 4.5, extracellular isoform, activity resistant to EDTA inhibition	Mycothermus thermophilus
12.3	-	40°C, pH 4.5, intracellular isoform, activity sensitive to EDTA inhibition	Trichocladium griseum
12.4	-	60°C, pH 4.5, extracellular isoform, activity sensitive to EDTA inhibition	Mycothermus thermophilus
16.5	-	40°C, pH 4.5, intracellular isoform, activity resistant to EDTA inhibition	Trichocladium griseum
18.4	-	40°C, pH 6.5, intracellular isoform, activity sensitive to EDTA inhibition	Trichocladium griseum
19.5	-	40°C, pH 6.5, intracellular isoform, activity sensitive to EDTA inhibition	Mycothermus thermophilus
25.6	-	40°C, pH 4.5, intracellular isoform, activity resistant to EDTA inhibition	Mycothermus thermophilus
26.2	-	60°C, pH 4.5, intracellular isoform, activity sensitive to EDTA inhibition	Trichocladium griseum
27	-	40°C, pH 6.5, intracellular isoform, activity resistant to EDTA inhibition	Trichocladium griseum
31.5	-	40°C, pH 4.5, extracellular isoform, activity resistant to EDTA inhibition	Trichocladium griseum
31.5	-	60°C, pH 4.5, extracellular isoform, activity sensitive to EDTA inhibition	Trichocladium griseum
42.2	-	40°C, pH 6.5, extracellular isoform, activity resistant to EDTA inhibition	Mycothermus thermophilus
46	-	40°C, pH 4.5, intracellular isoform, activity resistant to EDTA inhibition	Trichocladium griseum
48.6	-	40°C, pH 6.5, intracellular isoform, activity resistant to EDTA inhibition	Mycothermus thermophilus
49.6	-	60°C, pH 4.5, intracellular isoform, activity sensitive to EDTA inhibition	Mycothermus thermophilus
76.5	-	60°C, pH 6.5, intracellular isoform, activity sensitive to EDTA inhibition	Mycothermus thermophilus
76.8	-	60°C, pH 6.5, intracellular isoform, activity sensitive to EDTA inhibition	Trichocladium griseum
95.5	-	40°C, pH 6.5, extracellular isoform, activity sensitive to EDTA inhibition	Mycothermus thermophilus
100	-	40°C, pH 6.5, intracellular isoform, activity resistant to EDTA inhibition	Trichocladium griseum
113.4	-	40°C, pH 6.5, extracellular isoform, activity resistant to EDTA inhibition	Mycothermus thermophilus
154	-	60°C, pH 6.5, extracellular isoform, activity sensitive to EDTA inhibition	Mycothermus thermophilus
184	-	40°C, pH 6.5, extracellular isoform, activity resistant to EDTA inhibition	Trichocladium griseum
195.5	-	40°C, pH 6.5, extracellular isoform, activity sensitive to EDTA inhibition	Trichocladium griseum
274.8	-	60°C, pH 6.5, extracellular isoform, activity sensitive to EDTA inhibition	Trichocladium griseum
389	-	40°C, pH 6.5, extracellular isoform, activity resistant to EDTA inhibition	Trichocladium griseum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha,alpha-trehalose + H2O	-	Mycothermus thermophilus	2 D-glucose	-	?
alpha,alpha-trehalose + H2O	-	Trichocladium griseum	2 D-glucose	-	?

pH Range

pH Minimum	pH Maximum	Comment	Organism
4.5	-	at pH 4.5, enzyme is neither activated by calcium nor inhibited by EDTA or ATP.	Mycothermus thermophilus
4.5	-	at pH 4.5, enzyme is neither activated by calcium nor inhibited by EDTA or ATP.	Trichocladium griseum
6.5	-	at p-h 6.5, enzyme is activated by calcium and inhibited by ATP	Mycothermus thermophilus
6.5	-	at p-h 6.5, enzyme is activated by calcium and inhibited by ATP	Trichocladium griseum