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Literature summary for 3.2.1.28 extracted from
- Purification and characterization of a trehalase-invertase enzyme with dual activity from Candida utilis (2012), Arch. Biochem. Biophys., 522, 90-99.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Citric acid | 36.16% residual activity at 5 mM | Cyberlindnera jadinii |  |
| EDTA | 18.18% residual activity at 5 mM | Cyberlindnera jadinii | |
| KH2PO4 | 10.32% residual activity at 200 mM | Cyberlindnera jadinii | |
| MgSO4 | 57.02% residual activity at 2 mM | Cyberlindnera jadinii | |
| ZnSO4 | 2.06% residual activity at 10 mM | Cyberlindnera jadinii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
3 * 57000, SDS-PAGE | Cyberlindnera jadinii |
| 175000 | - |
gel filtration | Cyberlindnera jadinii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha,alpha-trehalose + H2O | Cyberlindnera jadinii | - |
beta-D-glucose + alpha-D-glucose | - |
? | |
| additional information | Cyberlindnera jadinii | trehalose-invertase is specific for both sucrose and trehalose. The ratio between trehalase and invertase activity is 1:3.5 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyberlindnera jadinii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Biosuite DEAE column chromatography, and gel filtration | Cyberlindnera jadinii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.01 | - |
supernatant, at pH 7.0 and 30°C | Cyberlindnera jadinii |
| 0.405 | - |
after 40fold purification, at pH 7.0 and 30°C | Cyberlindnera jadinii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha,alpha-trehalose + H2O | - |
Cyberlindnera jadinii | beta-D-glucose + alpha-D-glucose | - |
? | |
| additional information | trehalose-invertase is specific for both sucrose and trehalose. The ratio between trehalase and invertase activity is 1:3.5 | Cyberlindnera jadinii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotrimer | 3 * 57000, SDS-PAGE | Cyberlindnera jadinii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NT-INV | - |
Cyberlindnera jadinii |
| trehalase-invertase | - |
Cyberlindnera jadinii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Cyberlindnera jadinii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
no activity after 15 min at 70°C | Cyberlindnera jadinii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Cyberlindnera jadinii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9 | no activity at pH values less than 4.5 or more than 9.0 | Cyberlindnera jadinii |
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Release 2023.1 (January 2023)
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