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Literature summary for 3.2.1.28 extracted from

  • Silva, M.C.; Ribeiro, A.F.; Terra, W.R.; Ferreira, C.
    Sequencing of Spodoptera frugiperda midgut trehalases and demonstration of secretion of soluble trehalase by midgut columnar cells (2009), Insect Mol. Biol., 18, 769-784.
    View publication on PubMed
Search for more literature for 3.2.1.28

Cloned(Commentary)

Cloned (Comment) Organism
isozyme SfTre1, cDNA library preparation, DNA and amino acid sequence determination and analysis, phylogenetic tree and sequence comparisons, expression in Escherichia coli strain BL21(DE3) Spodoptera frugiperda
isozyme SfTre2, cDNA library preparation, DNA and amino acid sequence determination and analysis, phylogenetic tree and sequence comparisons, expression in Escherichia coli strain BL21(DE3) Spodoptera frugiperda

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
 alpha,alpha-trehalose isozyme SfTre1 Spodoptera frugiperda
1.6
-
 alpha,alpha-trehalose isozyme SfTre2 Spodoptera frugiperda

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound, isozyme SfTre2 Spodoptera frugiperda 16020
-
additional information immunohistochemic localization study of isozyme SfTre1 Spodoptera frugiperda
-
-
soluble isozyme SfTre1 Spodoptera frugiperda
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 x * 67000, isozyme SfTre1, SDS-PAGE Spodoptera frugiperda

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha,alpha-trehalose + H2O Spodoptera frugiperda
-
 2 D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Spodoptera frugiperda B5ATV4 membrane-bound trehalase SfTre2; membrane-bound isozyme SfTre2
-
Spodoptera frugiperda Q0ZIF5 soluble trehalase SfTre1; soluble isozyme SfTre1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein membrane-bound trehalase.SfTre2 contains potential N-glycosylation sites Spodoptera frugiperda
glycoprotein soluble trehalase.SfTre1 contains potential N-glycosylation sites Spodoptera frugiperda

Source Tissue

Source Tissue Comment Organism Textmining
columnar cell midgut Spodoptera frugiperda
-
columnar cell midgut, isozyme SfTre1 is localized at the secretory vesicles and at the glycocalyx of columnar cells Spodoptera frugiperda
-
larva
-
 Spodoptera frugiperda
-
midgut columnar cells, distribution of membrane-bound trehalase, isozyme SfTre2, along Spodoptera frugiperda midgut cells, overview Spodoptera frugiperda
-
midgut columnar cells, distribution of soluble trehalase, isozyme SfTre1, along Spodoptera frugiperda midgut cells, overview Spodoptera frugiperda
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,alpha-trehalose + H2O
-
 Spodoptera frugiperda 2 D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 67000, isozyme SfTre1, SDS-PAGE Spodoptera frugiperda

Synonyms

Synonyms Comment Organism
Alpha,alpha-trehalose glucohydrolase
-
 Spodoptera frugiperda
membrane-bound SfTre2
-
 Spodoptera frugiperda
SfTre1
-
 Spodoptera frugiperda
soluble trehalase
-
 Spodoptera frugiperda

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
62
-
 solubilized membrane-bound isozyme SfTre2, half-life is 10 min Spodoptera frugiperda
62
-
 soluble isozyme SfTre1, completely stable Spodoptera frugiperda