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Literature summary for 3.2.1.28 extracted from

  • Yoshida, T.; Sakamoto, T.
    Water-stress induced trehalose accumulation and control of trehalase in the cyanobacterium Nostoc punctiforme IAM M-15 (2009), J. Gen. Appl. Microbiol., 55, 135-145.
    View publication on PubMed
Search for more literature for 3.2.1.28

Cloned(Commentary)

Cloned (Comment) Organism
gene treH is organized in a gene cluster with the other two genes of the trehalose metabolism pathway, treZ and treY encoding malto-oligosyltrehalose trehalohydrolase and maltooligosyltrehalose synthase, DNA and amino acid sequence determination and analysis, overview. Expression of the N-terminally His-tagged active enzyme in Escherichia coli Nostoc punctiforme

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 over 90% inhibition at 10 mM Nostoc punctiforme
MgCl2 over 90% inhibition at 10 mM Nostoc punctiforme
MgSO4 over 90% inhibition at 20 mM Nostoc punctiforme
MnCl2 over 90% inhibition at 10 mM Nostoc punctiforme
additional information the enzyme activity is reduced under water-stress Nostoc punctiforme
NaCl strong inhibition at 10 mM, treatment of cells leads to accumulation of trehalose Nostoc punctiforme
Tris almost complete inhibition at 5 mM Nostoc punctiforme

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
18
-
 trehalose pH 7.5, 37°C, recombinant enzyme Nostoc punctiforme

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 x * 52000, recombinant His-tagged active enzyme, SDS-PAGE Nostoc punctiforme

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Nostoc punctiforme mechanism of trehalose accumulation in response to desiccation and salt stress, overview ?
-
 ?
additional information Nostoc punctiforme IAM M-15 mechanism of trehalose accumulation in response to desiccation and salt stress, overview ?
-
 ?
trehalose + H2O Nostoc punctiforme
-
 2 D-glucose
-
 ?
trehalose + H2O Nostoc punctiforme IAM M-15
-
 2 D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Nostoc punctiforme B6ZIV1 gene treH
-
Nostoc punctiforme IAM M-15 B6ZIV1 gene treH
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme by His affinity chromatography to homogeneity Nostoc punctiforme

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 determination of trehalose contents in differently treated cells, overview Nostoc punctiforme

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information mechanism of trehalose accumulation in response to desiccation and salt stress, overview Nostoc punctiforme ?
-
 ?
additional information mechanism of trehalose accumulation in response to desiccation and salt stress, overview Nostoc punctiforme IAM M-15 ?
-
 ?
trehalose + H2O
-
 Nostoc punctiforme 2 D-glucose
-
 ?
trehalose + H2O
-
 Nostoc punctiforme IAM M-15 2 D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 52000, recombinant His-tagged active enzyme, SDS-PAGE Nostoc punctiforme

Synonyms

Synonyms Comment Organism
trehalase
-
 Nostoc punctiforme

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Nostoc punctiforme

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Nostoc punctiforme

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the last step in the trehalose metabolism and is the key enzyme controlling the pathway in response to water conditions Nostoc punctiforme