KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
trehalose | soluble trehalase P II, inacetate buffer, pH 5.5, at 30°C | Bombyx mori | |
1.56 | - |
trehalose | soluble trehalase P I, in acetate buffer, pH 5.5, at 30°C | Bombyx mori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73000 | - |
soluble trehalase P I, SDS-PAGE | Bombyx mori |
140000 | - |
soluble trehalase P II, SDS-PAGE | Bombyx mori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sephacryl S-300 gel filtration, DEAE-cellulofine column chromatography, and Con A-Sepharose column chromatography | Bombyx mori |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
egg | - |
Bombyx mori | - |
embryo | - |
Bombyx mori | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
soluble trehalase P I from supernatant, in acetate buffer, pH 5.5, at 30°C | Bombyx mori |
0.01 | - |
soluble trehalase P II from supernatant, in acetate buffer, pH 5.5, at 30°C | Bombyx mori |
1.03 | - |
soluble trehalase P I after 86.9fold purification, in acetate buffer, pH 5.5, at 30°C | Bombyx mori |
2.9 | - |
soluble trehalase P II after 245.7fold purification, in acetate buffer, pH 5.5, at 30°C | Bombyx mori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trehalose + H2O | - |
Bombyx mori | D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acid trehalase | - |
Bombyx mori |
alpha,alpha glucoside 1-glucohydrolase | - |
Bombyx mori |
soluble P I type trehalase | - |
Bombyx mori |
soluble P II type trehalase | - |
Bombyx mori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | soluble trehalase P I | Bombyx mori |
50 | - |
above 50°C, soluble trehalase P I | Bombyx mori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
soluble trehalase P II | Bombyx mori |
6.5 | - |
soluble trehalase P I | Bombyx mori |