Application | Comment | Organism |
---|---|---|
biotechnology | immobilization of the crude enzyme on chitin resulting in stable, specific, and reusable reactors for application in other biotechnological processes, the immobilized enzyme shows no significant loss of activity being reused 10times and stored at 10°C, in 50 mM sodium maleate, pH 6.0, for 55 days | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
overexpression of the enzyme in strain Mph2 from plasmid pTRE11 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
alpha,alpha-trehalose | pH 5.5, 30°C, immobilized enzyme | Escherichia coli | |
1.32 | - |
alpha,alpha-trehalose | pH 5.5, 30°C, soluble enzyme | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Escherichia coli | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
x * 58000, recombinant enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli Mph2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
preparation of periplasmic fraction of recombinant Escherichia coli | Escherichia coli |
Storage Stability | Organism |
---|---|
immobilized crude recombinant enzyme on chitin shows no significant loss of activity being reused 10times and stored at 10°C, in 50 mM sodium maleate, pH 6.0, for 55 days | Escherichia coli |
Subunits | Comment | Organism |
---|---|---|
? | x * 58000, recombinant enzyme | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
trehalase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
immobilized enzyme | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
immobilized enzyme | Escherichia coli |