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Literature summary for 3.2.1.28 extracted from

  • Lucio-Eterovic, A.K.B.; Jorge, J.A.; Polizeli, M.de L.T.M.; Terenzi, H.F.
    Biochemical characterisation of the trehalase of thermophilic fungi: an enzyme with mixed properties of neutral and acid trehalase (2005), Biochim. Biophys. Acta, 1723, 201-207.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline inhibits the neutral trehalase in presence of 5 mM trehalose [Humicola] grisea var. thermoidea
1,10-phenanthroline inhibits the acid trehalase, inhibits the neutral trehalase in presence of 5 mM trehalose Mycothermus thermophilus
ATP
-
[Humicola] grisea var. thermoidea
ATP
-
Mycothermus thermophilus
ATP
-
Thermochaetoides thermophila
EDTA
-
[Humicola] grisea var. thermoidea
EDTA
-
Mycothermus thermophilus
EDTA
-
Thermochaetoides thermophila
gentiobiose slight inhibition of acid trehalase [Humicola] grisea var. thermoidea
gentiobiose slight inhibition of acid trehalase Mycothermus thermophilus
metal chelators
-
[Humicola] grisea var. thermoidea
metal chelators
-
Mycothermus thermophilus
metal chelators
-
Thermochaetoides thermophila
methyl-alpha-glucoside slight inhibition of acid trehalase [Humicola] grisea var. thermoidea
methyl-alpha-glucoside slight inhibition of acid trehalase Mycothermus thermophilus
phloretin inhibits the acid trehalase, inhibits the neutral trehalase in presence of 20 mM trehalose [Humicola] grisea var. thermoidea
phloretin inhibits the acid trehalase, inhibits the neutral trehalase in presence of 20 mM trehalose Mycothermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km of neutral and acid trehalase at different temperatures and pH values Mycothermus thermophilus
additional information
-
additional information Km of neutral and acid trehalase at different temperatures and pH values [Humicola] grisea var. thermoidea

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular acid trehalase Thermochaetoides thermophila
-
-
extracellular acid trehalase Mycothermus thermophilus
-
-
extracellular acid trehalase [Humicola] grisea var. thermoidea
-
-
intracellular neutral trehalase Thermochaetoides thermophila 5622
-
intracellular neutral trehalase Mycothermus thermophilus 5622
-
intracellular neutral trehalase [Humicola] grisea var. thermoidea 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Thermochaetoides thermophila
Ca2+ activates Mycothermus thermophilus
Ca2+ activates, dependent on pH [Humicola] grisea var. thermoidea
gentiobiose activates the neutral trehalase Mycothermus thermophilus
gentiobiose activates the neutral trehalase [Humicola] grisea var. thermoidea
Mn2+ activates Thermochaetoides thermophila
Mn2+ activates Mycothermus thermophilus
Mn2+ activates [Humicola] grisea var. thermoidea
phloretin activates the neutral trehalase in presence of 5 mM trehalose Mycothermus thermophilus
phloretin activates the neutral trehalase in presence of 5 mM trehalose [Humicola] grisea var. thermoidea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha,alpha-trehalose + H2O Thermochaetoides thermophila
-
2 D-glucose
-
?
alpha,alpha-trehalose + H2O Mycothermus thermophilus
-
2 D-glucose
-
?
alpha,alpha-trehalose + H2O [Humicola] grisea var. thermoidea
-
2 D-glucose
-
?
alpha,alpha-trehalose + H2O Mycothermus thermophilus 77.7.8
-
2 D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Mycothermus thermophilus
-
isolated from indian soil
-
Mycothermus thermophilus 77.7.8
-
isolated from indian soil
-
Thermochaetoides thermophila
-
-
-
[Humicola] grisea var. thermoidea
-
isolated from brazilian soil
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein acid trehalase Thermochaetoides thermophila
glycoprotein acid trehalase Mycothermus thermophilus
glycoprotein acid trehalase [Humicola] grisea var. thermoidea

Purification (Commentary)

Purification (Comment) Organism
-
Mycothermus thermophilus
-
[Humicola] grisea var. thermoidea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activities of neutral and acid trehalase at different pH values and temperatures Mycothermus thermophilus
additional information
-
activities of neutral and acid trehalase at different pH values and temperatures [Humicola] grisea var. thermoidea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,alpha-trehalose + H2O
-
Thermochaetoides thermophila 2 D-glucose
-
?
alpha,alpha-trehalose + H2O
-
Mycothermus thermophilus 2 D-glucose
-
?
alpha,alpha-trehalose + H2O
-
[Humicola] grisea var. thermoidea 2 D-glucose
-
?
alpha,alpha-trehalose + H2O
-
Mycothermus thermophilus 77.7.8 2 D-glucose
-
?

Synonyms

Synonyms Comment Organism
acid trehalase
-
Thermochaetoides thermophila
acid trehalase
-
Mycothermus thermophilus
acid trehalase
-
[Humicola] grisea var. thermoidea
neutral trehalase
-
Thermochaetoides thermophila
neutral trehalase
-
Mycothermus thermophilus
neutral trehalase
-
[Humicola] grisea var. thermoidea
trehalase
-
Thermochaetoides thermophila
trehalase
-
Mycothermus thermophilus
trehalase
-
[Humicola] grisea var. thermoidea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
the acid trehalase shows a higher temperature optimum than the neutral trehalase Thermochaetoides thermophila
60
-
the acid trehalase shows a higher temperature optimum than the neutral trehalase Mycothermus thermophilus
60
-
the acid trehalase shows a higher temperature optimum than the neutral trehalase [Humicola] grisea var. thermoidea

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60
-
[Humicola] grisea var. thermoidea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
the acid trehalase is more thermostable than the neutral trehalase Thermochaetoides thermophila
additional information
-
the acid trehalase is more thermostable than the neutral trehalase Mycothermus thermophilus
additional information
-
the acid trehalase is more thermostable than the neutral trehalase [Humicola] grisea var. thermoidea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
acid trehalase Mycothermus thermophilus
4.5
-
acid trehalase [Humicola] grisea var. thermoidea
6.5
-
neutral trehalase Mycothermus thermophilus
6.5
-
neutral trehalase [Humicola] grisea var. thermoidea

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7
-
Mycothermus thermophilus
4.5 7
-
[Humicola] grisea var. thermoidea