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Literature summary for 3.2.1.28 extracted from

  • Lee, J.H.; Tsuji, M.; Nakamura, M.; Nishimoto, M.; Okuyama, M.; Mori, H.; Kimura, A.; Matsui, H.; Chiba, S.
    Purification and identification of the essential ionizable groups of honeybee, Apis mellifera L., trehalase (2001), Biosci. Biotechnol. Biochem., 65, 2657-2665.
    View publication on PubMed
Search for more literature for 3.2.1.28

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.66
-
 alpha,alpha-trehalose
-
 Apis mellifera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000 69000 gel filtration Apis mellifera
69000
-
 1 * 69000 Apis mellifera

Organism

Organism UniProt Comment Textmining
Apis mellifera
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 3.1% carbohydrate Apis mellifera

Purification (Commentary)

Purification (Comment) Organism
-
 Apis mellifera

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
427
-
-
 Apis mellifera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,alpha-trehalose + H2O inverting-type enzyme Apis mellifera alpha-D-glucopyranose + D-glucose
-
 ?
additional information no activity with alpha,beta-trehalose and 6-beta-glucosyl-trehalose Apis mellifera ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 69000 Apis mellifera

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 15 min, stable up to Apis mellifera
50
-
 15 min, about 35% of maximal activity Apis mellifera
55
-
 15 min, about 25% of maximal activity Apis mellifera
60
-
 15 min, complete inactivation Apis mellifera

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information the molecular activity is 86.2 per s Apis mellifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
-
 Apis mellifera

pH Range

pH Minimum pH Maximum Comment Organism
4.8 8.5 pH 4.9: about 50% of maximal activity, pH 8.5: about 40% of maximal activity Apis mellifera

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 12 4°C, 24 h, stable Apis mellifera