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Literature summary for 3.2.1.26 extracted from

  • Xu, Z.; Liu, C.; Cai, S.; Zhang, L.; Xiong, Z.
    Heterologous expression and comparative characterization of vacuolar invertases from Cu-tolerant and non-tolerant populations of Elsholtzia haichowensis (2015), Plant Cell Rep., 34, 1781-1790 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
genes from the Cu-tolerant population (EhCvINV) and non-tolerant population (EhNvINV) are heterologously expressed in Pichia pastoris Elsholtzia haichowensis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Elsholtzia haichowensis
Cd2+
-
Elsholtzia haichowensis
Co2+
-
Elsholtzia haichowensis
Cu2+
-
Elsholtzia haichowensis
Hg2+
-
Elsholtzia haichowensis
Mg2+
-
Elsholtzia haichowensis
Ni2+
-
Elsholtzia haichowensis
Pb2+
-
Elsholtzia haichowensis
Zn2+
-
Elsholtzia haichowensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.68
-
sucrose 30°C, pH 5.0 Elsholtzia haichowensis
2.77
-
sucrose 30°C, pH 5.0 Elsholtzia haichowensis

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuole
-
Elsholtzia haichowensis 5773
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
SDS-PAGE Elsholtzia haichowensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + H2O Elsholtzia haichowensis
-
beta-D-fructose + alpha-D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Elsholtzia haichowensis K4P5F3
-
-
Elsholtzia haichowensis K4P7S0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Elsholtzia haichowensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information vacuolar invertases from Cu-tolerant and non-tolerant populations of Elsholtzia haichowensis have similar enzyme properties, and the enzyme protein divergences contribute little to the varied vacuolar invertase activities between the contrasting populations Elsholtzia haichowensis ?
-
?
sucrose + H2O
-
Elsholtzia haichowensis beta-D-fructose + alpha-D-glucose
-
?

Subunits

Subunits Comment Organism
? x * 90000, SDS-PAGE Elsholtzia haichowensis

Synonyms

Synonyms Comment Organism
vacuolar invertase CvINV enzyme from from the Cu-tolerant population Elsholtzia haichowensis
vacuolar invertase NvINV enzyme from from the non-Cu-tolerant population Elsholtzia haichowensis
VIN
-
Elsholtzia haichowensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Elsholtzia haichowensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 40°C: about 60% of maximal activity, 80°C: about 10% of maximal activity Elsholtzia haichowensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
429.24
-
sucrose 30°C, pH 5.0 Elsholtzia haichowensis
450.9
-
sucrose 30°C, pH 5.0 Elsholtzia haichowensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5
-
Elsholtzia haichowensis

pH Range

pH Minimum pH Maximum Comment Organism
4 6 pH 4.0: about 80% of maximal activity, pH 6.0: about 40% of maximal activity Elsholtzia haichowensis
4 6 pH 4.0: about 80% of maximal activity, pH 6.0: about 45% of maximal activity Elsholtzia haichowensis

pI Value

Organism Comment pI Value Maximum pI Value
Elsholtzia haichowensis calculated from sequence
-
4.88

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
160.19
-
sucrose 30°C, pH 5.0 Elsholtzia haichowensis
163.4
-
sucrose 30°C, pH 5.0 Elsholtzia haichowensis