Cloned (Comment) | Organism |
---|---|
genes from the Cu-tolerant population (EhCvINV) and non-tolerant population (EhNvINV) are heterologously expressed in Pichia pastoris | Elsholtzia haichowensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Elsholtzia haichowensis | |
Cd2+ | - |
Elsholtzia haichowensis | |
Co2+ | - |
Elsholtzia haichowensis | |
Cu2+ | - |
Elsholtzia haichowensis | |
Hg2+ | - |
Elsholtzia haichowensis | |
Mg2+ | - |
Elsholtzia haichowensis | |
Ni2+ | - |
Elsholtzia haichowensis | |
Pb2+ | - |
Elsholtzia haichowensis | |
Zn2+ | - |
Elsholtzia haichowensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.68 | - |
sucrose | 30°C, pH 5.0 | Elsholtzia haichowensis | |
2.77 | - |
sucrose | 30°C, pH 5.0 | Elsholtzia haichowensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
vacuole | - |
Elsholtzia haichowensis | 5773 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
SDS-PAGE | Elsholtzia haichowensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + H2O | Elsholtzia haichowensis | - |
beta-D-fructose + alpha-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Elsholtzia haichowensis | K4P5F3 | - |
- |
Elsholtzia haichowensis | K4P7S0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Elsholtzia haichowensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | vacuolar invertases from Cu-tolerant and non-tolerant populations of Elsholtzia haichowensis have similar enzyme properties, and the enzyme protein divergences contribute little to the varied vacuolar invertase activities between the contrasting populations | Elsholtzia haichowensis | ? | - |
? | |
sucrose + H2O | - |
Elsholtzia haichowensis | beta-D-fructose + alpha-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 90000, SDS-PAGE | Elsholtzia haichowensis |
Synonyms | Comment | Organism |
---|---|---|
vacuolar invertase CvINV | enzyme from from the Cu-tolerant population | Elsholtzia haichowensis |
vacuolar invertase NvINV | enzyme from from the non-Cu-tolerant population | Elsholtzia haichowensis |
VIN | - |
Elsholtzia haichowensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Elsholtzia haichowensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | 40°C: about 60% of maximal activity, 80°C: about 10% of maximal activity | Elsholtzia haichowensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
429.24 | - |
sucrose | 30°C, pH 5.0 | Elsholtzia haichowensis | |
450.9 | - |
sucrose | 30°C, pH 5.0 | Elsholtzia haichowensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5 | - |
Elsholtzia haichowensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | pH 4.0: about 80% of maximal activity, pH 6.0: about 40% of maximal activity | Elsholtzia haichowensis |
4 | 6 | pH 4.0: about 80% of maximal activity, pH 6.0: about 45% of maximal activity | Elsholtzia haichowensis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Elsholtzia haichowensis | calculated from sequence | - |
4.88 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
160.19 | - |
sucrose | 30°C, pH 5.0 | Elsholtzia haichowensis | |
163.4 | - |
sucrose | 30°C, pH 5.0 | Elsholtzia haichowensis |