Literature summary for 3.2.1.26 extracted from

Xie, J.; Cai, K.; Hu, H.X.; Jiang, Y.L.; Yang, F.; Hu, P.F.; Cao, D.D.; Li, W.F.; Chen, Y.; Zhou, C.Z.
Structural analysis of the catalytic mechanism and substrate specificity of Anabaena akaline invertase InvA reveals a novel glucosidase (2016), J. Biol. Chem., 291, 25667-25677 .

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of a truncated version of InvA (residues Lys9-Thr460)	Nostoc sp. PCC 7120 = FACHB-418

Crystallization (Commentary)

Crystallization (Comment)	Organism
partial proteolysis and multiple sequence alignment indicate that both N and C termini are somewhat unstructured. Thus, we overexpressed and purified a truncated version of InvA (residues Lys9-Thr460) and successfully optimized its crystal co-crystallized with 200 mM fructose, the 2.11 A SeMet-substituted crystal of which was used for structure determination. In the sucrose-complexed structure (InvA-Suc), which has a space group of C2221, each asymmetric unit contains three molecules of InvA	Nostoc sp. PCC 7120 = FACHB-418

Crystallization (Comment)

Organism

partial proteolysis and multiple sequence alignment indicate that both N and C termini are somewhat unstructured. Thus, we overexpressed and purified a truncated version of InvA (residues Lys9-Thr460) and successfully optimized its crystal co-crystallized with 200 mM fructose, the 2.11 A SeMet-substituted crystal of which was used for structure determination. In the sucrose-complexed structure (InvA-Suc), which has a space group of C2221, each asymmetric unit contains three molecules of InvA

Nostoc sp. PCC 7120 = FACHB-418

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
30.7	-	sucrose	pH and temperature not specified in the publication	Nostoc sp. PCC 7120 = FACHB-418

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sucrose + H2O	Nostoc sp. PCC 7120 = FACHB-418	the enzyme plays a key role in primary metabolism and plant development	beta-D-fructose + alpha-D-glucose	-	?

Organism

Organism	UniProt	Comment	Textmining
Nostoc sp. PCC 7120 = FACHB-418	Q8YWS9	-	-

Purification (Commentary)

Purification (Comment)	Organism
truncated version of InvA (residues Lys9-Thr460)	Nostoc sp. PCC 7120 = FACHB-418

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sucrose + H2O	-	Nostoc sp. PCC 7120 = FACHB-418	beta-D-fructose + alpha-D-glucose	-	?
sucrose + H2O	the enzyme plays a key role in primary metabolism and plant development	Nostoc sp. PCC 7120 = FACHB-418	beta-D-fructose + alpha-D-glucose	-	?

Subunits

Subunits	Comment	Organism
hexamer	invertase InvA exists as a hexamer in both crystal and solution	Nostoc sp. PCC 7120 = FACHB-418

Synonyms

Synonyms	Comment	Organism
alkaline invertase InvA	-	Nostoc sp. PCC 7120 = FACHB-418

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.9	-	-	Nostoc sp. PCC 7120 = FACHB-418

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	9	pH 7.0: about 40% of maximal activity, pH 9.0: about 60% of maximal activity	Nostoc sp. PCC 7120 = FACHB-418

General Information

General Information	Comment	Organism
physiological function	the enzyme plays a key role in primary metabolism and plant development	Nostoc sp. PCC 7120 = FACHB-418