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Literature summary for 3.2.1.26 extracted from

  • Belaz, S.; Rattier, T.; Lafite, P.; Moreau, P.; Routier, F.H.; Robert-Gangneux, F.; Gangneux, J.P.; Daniellou, R.
    Identification, biochemical characterization, and in-vivo expression of the intracellular invertase BfrA from the pathogenic parasite Leishmania major (2015), Carbohydr. Res., 415, 31-38 .
    View publication on PubMed

Application

Application Comment Organism
pharmacology putative target for antileishmanian drug development Leishmania donovani
pharmacology putative target for antileishmanian drug development Leishmania major
pharmacology putative target for antileishmanian drug development Leishmania braziliensis

Cloned(Commentary)

Cloned (Comment) Organism
heterologous overexpression in both Escherichia coli and Leishmania tarentolae Leishmania major

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9
-
inulin 37°C, pH 7.5 Leishmania major
141
-
raffinose 37°C, pH 7.5 Leishmania major
152
-
sucrose 37°C, pH 7.5 Leishmania major
168
-
stachyose 37°C, pH 7.5 Leishmania major

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Leishmania braziliensis A4HCW0
-
-
Leishmania donovani
-
-
-
Leishmania major Q4QB75
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Leishmania major

Source Tissue

Source Tissue Comment Organism Textmining
amastigote expressed to a higher extent in promastigotes than in amastigotes Leishmania donovani
-
amastigote expressed to a higher extent in promastigotes than in amastigotes Leishmania major
-
amastigote expressed to a higher extent in promastigotes than in amastigotes Leishmania braziliensis
-
promastigote expressed to a higher extent in promastigotes than in amastigotes. Induction increases over time and the strongest mRNA induction is observed at day 7, suggesting that invertase expression is highly expressed by promastigotes at stationary phase Leishmania donovani
-
promastigote expressed to a higher extent in promastigotes than in amastigotes. Induction increases over time and the strongest mRNA induction is observed at day 7, suggesting that invertase expression is highly expressed by promastigotes at stationary phase Leishmania major
-
promastigote expressed to a higher extent in promastigotes than in amastigotes. Induction increases over time and the strongest mRNA induction is observed at day 7, suggesting that invertase expression is highly expressed by promastigotes at stationary phase Leishmania braziliensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-kestose + H2O 5.6% compared to the activity with sucrose Leishmania major D-fructose + ?
-
?
inulin + H2O 10.9% compared to the activity with sucrose Leishmania major D-fructose + D-glucose + fructooligosaccharides
-
?
additional information no hydrolysis when using 4-nitrophenyl-alpha-D-glucopyranose as substrate Leishmania major ?
-
?
raffinose + H2O 109% compared to the activity with sucrose Leishmania major beta-D-fructose + alpha-D-melibiose
-
?
stachyose + H2O 297% compared to the activity with sucrose Leishmania major D-fructose + alpha-D-Gal-(1->6)-alpha-D-Gal-(1->6)-alpha-D-Gal
-
?
sucrose
-
Leishmania major beta-D-fructose + alpha-D-glucose
-
?

Synonyms

Synonyms Comment Organism
BfrA
-
Leishmania donovani
BfrA
-
Leishmania major

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Leishmania major

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 20°C: about 60% of maximal activity, 50°C: about 45% of maximal activity Leishmania major

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2
-
inulin 37°C, pH 7.5 Leishmania major
114
-
sucrose 37°C, pH 7.5 Leishmania major
118
-
raffinose 37°C, pH 7.5 Leishmania major
409
-
stachyose 37°C, pH 7.5 Leishmania major

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Leishmania major

pH Range

pH Minimum pH Maximum Comment Organism
5 7.9 pH 5.0: about 75% of maximal activity, pH 7.0: about 75% of maximal activity Leishmania major

Expression

Organism Comment Expression
Leishmania major expressed to a higher extent in promastigotes than in amastigotes up
Leishmania braziliensis expressed to a higher extent in promastigotes than in amastigotes up

General Information

General Information Comment Organism
physiological function the enzyme could be important for the development and growth of the parasite in the sandfly gut Leishmania donovani
physiological function the enzyme could be important for the development and growth of the parasite in the sandfly gut Leishmania major
physiological function the enzyme could be important for the development and growth of the parasite in the sandfly gut Leishmania braziliensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.75
-
sucrose 37°C, pH 7.5 Leishmania major
0.86
-
raffinose 37°C, pH 7.5 Leishmania major
2.4
-
stachyose 37°C, pH 7.5 Leishmania major
4.6
-
inulin 37°C, pH 7.5 Leishmania major