Application | Comment | Organism |
---|---|---|
pharmacology | putative target for antileishmanian drug development | Leishmania donovani |
pharmacology | putative target for antileishmanian drug development | Leishmania major |
pharmacology | putative target for antileishmanian drug development | Leishmania braziliensis |
Cloned (Comment) | Organism |
---|---|
heterologous overexpression in both Escherichia coli and Leishmania tarentolae | Leishmania major |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
inulin | 37°C, pH 7.5 | Leishmania major | |
141 | - |
raffinose | 37°C, pH 7.5 | Leishmania major | |
152 | - |
sucrose | 37°C, pH 7.5 | Leishmania major | |
168 | - |
stachyose | 37°C, pH 7.5 | Leishmania major |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania braziliensis | A4HCW0 | - |
- |
Leishmania donovani | - |
- |
- |
Leishmania major | Q4QB75 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Leishmania major |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amastigote | expressed to a higher extent in promastigotes than in amastigotes | Leishmania donovani | - |
amastigote | expressed to a higher extent in promastigotes than in amastigotes | Leishmania major | - |
amastigote | expressed to a higher extent in promastigotes than in amastigotes | Leishmania braziliensis | - |
promastigote | expressed to a higher extent in promastigotes than in amastigotes. Induction increases over time and the strongest mRNA induction is observed at day 7, suggesting that invertase expression is highly expressed by promastigotes at stationary phase | Leishmania donovani | - |
promastigote | expressed to a higher extent in promastigotes than in amastigotes. Induction increases over time and the strongest mRNA induction is observed at day 7, suggesting that invertase expression is highly expressed by promastigotes at stationary phase | Leishmania major | - |
promastigote | expressed to a higher extent in promastigotes than in amastigotes. Induction increases over time and the strongest mRNA induction is observed at day 7, suggesting that invertase expression is highly expressed by promastigotes at stationary phase | Leishmania braziliensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-kestose + H2O | 5.6% compared to the activity with sucrose | Leishmania major | D-fructose + ? | - |
? | |
inulin + H2O | 10.9% compared to the activity with sucrose | Leishmania major | D-fructose + D-glucose + fructooligosaccharides | - |
? | |
additional information | no hydrolysis when using 4-nitrophenyl-alpha-D-glucopyranose as substrate | Leishmania major | ? | - |
? | |
raffinose + H2O | 109% compared to the activity with sucrose | Leishmania major | beta-D-fructose + alpha-D-melibiose | - |
? | |
stachyose + H2O | 297% compared to the activity with sucrose | Leishmania major | D-fructose + alpha-D-Gal-(1->6)-alpha-D-Gal-(1->6)-alpha-D-Gal | - |
? | |
sucrose | - |
Leishmania major | beta-D-fructose + alpha-D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BfrA | - |
Leishmania donovani |
BfrA | - |
Leishmania major |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Leishmania major |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | 20°C: about 60% of maximal activity, 50°C: about 45% of maximal activity | Leishmania major |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
inulin | 37°C, pH 7.5 | Leishmania major | |
114 | - |
sucrose | 37°C, pH 7.5 | Leishmania major | |
118 | - |
raffinose | 37°C, pH 7.5 | Leishmania major | |
409 | - |
stachyose | 37°C, pH 7.5 | Leishmania major |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Leishmania major |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.9 | pH 5.0: about 75% of maximal activity, pH 7.0: about 75% of maximal activity | Leishmania major |
Organism | Comment | Expression |
---|---|---|
Leishmania major | expressed to a higher extent in promastigotes than in amastigotes | up |
Leishmania braziliensis | expressed to a higher extent in promastigotes than in amastigotes | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme could be important for the development and growth of the parasite in the sandfly gut | Leishmania donovani |
physiological function | the enzyme could be important for the development and growth of the parasite in the sandfly gut | Leishmania major |
physiological function | the enzyme could be important for the development and growth of the parasite in the sandfly gut | Leishmania braziliensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.75 | - |
sucrose | 37°C, pH 7.5 | Leishmania major | |
0.86 | - |
raffinose | 37°C, pH 7.5 | Leishmania major | |
2.4 | - |
stachyose | 37°C, pH 7.5 | Leishmania major | |
4.6 | - |
inulin | 37°C, pH 7.5 | Leishmania major |