Cloned (Comment) | Organism |
---|---|
expressed in methylotrophic yeast Pichia pastoris | Elsholtzia haichowensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, 25% loss of activity | Elsholtzia haichowensis | |
Cd2+ | 5 mM, 63% loss of activity | Elsholtzia haichowensis | |
Co2+ | 5 mM, 56% loss of activity | Elsholtzia haichowensis | |
Cu2+ | 5 mM, 23% loss of activity | Elsholtzia haichowensis | |
Hg2+ | 5 mM, complete loss of activity | Elsholtzia haichowensis | |
Mg2+ | 5 mM, 23% loss of activity | Elsholtzia haichowensis | |
Ni2+ | 5 mM, 52% loss of activity | Elsholtzia haichowensis | |
Pb2+ | 5 mM, 48% loss of activity | Elsholtzia haichowensis | |
Zn2+ | 5 mM, 38% loss of activity | Elsholtzia haichowensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.282 | - |
sucrose | 30°C, pH 5.0 | Elsholtzia haichowensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Elsholtzia haichowensis | 5618 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59792 | - |
mature enzyme without signal peptide, calculated from sequence | Elsholtzia haichowensis |
62500 | - |
calculated from sequence | Elsholtzia haichowensis |
750800 | - |
SDS-PAGE | Elsholtzia haichowensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Elsholtzia haichowensis | A0A089N0W4 | copper tolerant population | - |
Purification (Comment) | Organism |
---|---|
- |
Elsholtzia haichowensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Elsholtzia haichowensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + H2O | - |
Elsholtzia haichowensis | beta-D-fructose + alpha-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 59792, mature enzyme without signal peptide, calculated from sequence | Elsholtzia haichowensis |
? | x * 750800, SDS-PAGE | Elsholtzia haichowensis |
Synonyms | Comment | Organism |
---|---|---|
FCWI | - |
Elsholtzia haichowensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Elsholtzia haichowensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | 30°C: about 35% of maximal activity, 60°C: about 90% of maximal activity | Elsholtzia haichowensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | recombinant enzyme shows drastic loss of activity from 60°C to 70°C | Elsholtzia haichowensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
119.4 | - |
sucrose | 30°C, pH 5.0 | Elsholtzia haichowensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Elsholtzia haichowensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 5 | pH 3.5: about 45% of maximal activity, pH 5.0: about 50% of maximal activity | Elsholtzia haichowensis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Elsholtzia haichowensis | calculated from sequence | - |
9.29 |
Elsholtzia haichowensis | mature enzyme without signal peptide | - |
9.33 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
423 | - |
sucrose | 30°C, pH 5.0 | Elsholtzia haichowensis |