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Literature summary for 3.2.1.26 extracted from

  • Liu, C.; Xu, Z.; Cai, S.; Zhang, L.; Xiong, Z.
    cDNA cloning, heterologous expression and characterization of a cell wall invertase from copper tolerant population of Elsholtzia haichowensis (2015), Biologia, 70, 1063-1069 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in methylotrophic yeast Pichia pastoris Elsholtzia haichowensis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 5 mM, 25% loss of activity Elsholtzia haichowensis
Cd2+ 5 mM, 63% loss of activity Elsholtzia haichowensis
Co2+ 5 mM, 56% loss of activity Elsholtzia haichowensis
Cu2+ 5 mM, 23% loss of activity Elsholtzia haichowensis
Hg2+ 5 mM, complete loss of activity Elsholtzia haichowensis
Mg2+ 5 mM, 23% loss of activity Elsholtzia haichowensis
Ni2+ 5 mM, 52% loss of activity Elsholtzia haichowensis
Pb2+ 5 mM, 48% loss of activity Elsholtzia haichowensis
Zn2+ 5 mM, 38% loss of activity Elsholtzia haichowensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.282
-
sucrose 30°C, pH 5.0 Elsholtzia haichowensis

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
Elsholtzia haichowensis 5618
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59792
-
mature enzyme without signal peptide, calculated from sequence Elsholtzia haichowensis
62500
-
calculated from sequence Elsholtzia haichowensis
750800
-
SDS-PAGE Elsholtzia haichowensis

Organism

Organism UniProt Comment Textmining
Elsholtzia haichowensis A0A089N0W4 copper tolerant population
-

Purification (Commentary)

Purification (Comment) Organism
-
Elsholtzia haichowensis

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Elsholtzia haichowensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + H2O
-
Elsholtzia haichowensis beta-D-fructose + alpha-D-glucose
-
?

Subunits

Subunits Comment Organism
? x * 59792, mature enzyme without signal peptide, calculated from sequence Elsholtzia haichowensis
? x * 750800, SDS-PAGE Elsholtzia haichowensis

Synonyms

Synonyms Comment Organism
FCWI
-
Elsholtzia haichowensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Elsholtzia haichowensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 30°C: about 35% of maximal activity, 60°C: about 90% of maximal activity Elsholtzia haichowensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 70 recombinant enzyme shows drastic loss of activity from 60°C to 70°C Elsholtzia haichowensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
119.4
-
sucrose 30°C, pH 5.0 Elsholtzia haichowensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Elsholtzia haichowensis

pH Range

pH Minimum pH Maximum Comment Organism
3.5 5 pH 3.5: about 45% of maximal activity, pH 5.0: about 50% of maximal activity Elsholtzia haichowensis

pI Value

Organism Comment pI Value Maximum pI Value
Elsholtzia haichowensis calculated from sequence
-
9.29
Elsholtzia haichowensis mature enzyme without signal peptide
-
9.33

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
423
-
sucrose 30°C, pH 5.0 Elsholtzia haichowensis